| 货号 | FAB3007G |
| 别名 | B and T lymphocyte associated; B and T lymphocyte attenuator; B- and T-lymphocyte attenuator; B- and T-lymphocyte-associated protein; BTLA1; CD272 antigen; CD272; FLJ16065; MGC129743 | 全称 | B- And T-Lymphocyte Attenuator |
| 反应种属 | Mouse |
| 应用 | Flow Cytometry(5 µL/106cells) |
| 目标/特异性 | Detects mouse BTLA in direct ELISAs and Western blots. In direct ELISAs and Western blots, less than 1% cross-reactivity with recombinant human BTLA is observed. |
| 使用方法 | Flow Cytometry: 5 µL/106cells |
| 来源 | The product is shipped with polar packs. Upon receipt, store it immediately at the temperature recommended below. |
| 产品组分 |
| 供应商 | R&D Systems |
| Entrez Gene IDs | 151888 (Human); 208154 (Mouse) |
| 纯化方式 | Antigen Affinity-purified |
| 免疫原 | Mouse myeloma cell line NS0-derived recombinant mouse BTLA Glu30-Gly176 (Pro41Glu, Thr45Asn, Thr47Lys, Gln52His, Arg55Trp, Gln63Glu, Cys85Trp) Accession # Q32MV9 |
| 生物活性 | Mouse |
| 标记 | Alexa Fluor 488 |
| 背景 | B- and T-lymphocyte Attenuator (BTLA), also known as CD272, is a 70 kDa, Ig-superfamily, type I transmembrane glycoprotein that is structurally similar to the CD28 family of T cell co-stimulatory or coinhibitory molecules (1‑3). Unlike CD28 family members, however, the BTLA extracellular Ig domain is an I-type rather than a V-type domain, and BTLA does not form homodimers (4). BTLA also differs from CD28 family members through the interaction of its Ig domain with the TNF superfamily member HVEM (herpesvirus entry mediator; TNFSF14) rather than with B7 family ligands (5). BTLA is a coinhibitory molecule expressed on T cells, B cells and, depending on the mouse strain, macrophages, dendritic and NK cells (6). Expression is low in naïve T cells and increased during antigen-specific induction of anergy. In B cells, BTLA is highest when cells are mature and naïve (6). BTLA apparently limits T cell numbers, since deletion of BTLA results in overproduction of T cells, especially CD8+ memory T cells that are hyper-responsive to TCR crosslinking (7). The 306 amino acid (aa) BTLA contains a 29 aa signal sequence, a 154 aa extracellular domain (ECD), a 21 aa transmembrane sequence, and a 102 aa cytoplasmic domain. There are two ITIM motifs and three Tyr phosphorylation sites in the cytoplasmic tail that mediate inhibitory signaling (8, 9). The binding of the BTLA to HVEM does not preclude additional binding of a mammalian stimulatory HVEM ligand, either LIGHT or lymphotoxin-alpha to the complex (4). At least three alleles varying by up to ten extracellular amino acids occur in different mouse strains (6). The ECD of C57BL/6 BTLA shows 51%, 77% and 40% aa identity to that of human, rat and canine BTLA, respectively. A splice variant lacking the Ig domain, termed BTLAs, has been reported (3). |
| 运输条件 | Blue Ice |
| 存放说明 | 4℃ |
| 参考文献 |
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Detection of BTLA in B220+ Mouse Splenocytes by Flow Cytometry. B220+ mouse splenocytes were stained with Goat Anti-Mouse BTLA Alexa Fluor® 488‑conjugated Antigen Affinity-purified Polyclonal Antibody (Catalog # FAB3007G, filled histogram) or isotype control antibody (Catalog # IC108G, open histogram). View our protocol for Staining Membrane-associated Proteins. |
