概述| 货号 | IC7794P |
| 别名 | PKB; RAC |
全称 | v-Akt Murine Thymoma Viral Oncogene Homolog | | 应用 | Intracellular Staining by Flow Cytometry(10 µL/106cells)
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| 目标/特异性 | Detects human and mouse Akt1, Akt2 and Akt3, when phosphorylated at S473, S474 and S472, respectively. |
| 使用方法 | Intracellular Staining by Flow Cytometry: 10 µL/106cells
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| 来源 | The product is shipped with polar packs. Upon receipt, store it immediately at the temperature recommended below. |
| 产品组分 | |
性能| 供应商 | R&D Systems |
| 纯化方式 | Protein A or G purified from hybridoma culture supernatant |
| 免疫原 | Phosphopeptide containing the human Akt (S473) site |
| 生物活性 | Human, Mouse |
| 标记 | Phycoerythrin |
| 背景 | Akt, also known as Protein Kinase B (PKB), is a central kinase in such diverse cellular processes as glucose uptake, cell cycle progression, and apoptosis. Three highly homologous members define the Akt family: Akt1 (PKB alpha ), Akt2 (PKB beta ), and Akt3 (PKB gamma ). All three Akts contain an amino-terminal pleckstrin homology domain, a central kinase domain, and a carboxyl-terminal regulatory domain. Akt1 is the most widely expressed family member and is frequently activated in a number of carcinomas, including breast, prostate, lung, pancreatic, liver, ovarian, and colorectal cancer. Akt1 is activated in a multistep process that involves the sequential phosphorylation of Thr450 by JNK kinases, Thr308 by PDK1, and Ser473 by PDK2 or mTORC2. Activated Akt1 phosphorylates a wide variety of cytosolic, nuclear, and mitochondrial substrates. Human Akt1 shares 98% aa sequence identity with mouse and rat Akt1. |
| 运输条件 | Blue Ice |
| 存放说明 | 4℃ |
| 参考文献 | - A Novel Pathway for TOR-independent Autophagy Regulation by Akt
- Akt Modulates TGF-beta Activities
- Apoptosis Intracellular Kinases
- Cancer Biomarkers
- Chemokine Receptor Intracellular Signaling
- Common gamma Chain Receptor Family
- Diabetic Peripheral Neuropathy
- GDNF Family
- Human Phospho-MAPK Array Kit
- ICC / IHC Images: Akt
- Interactive Pathway: Akt Signaling Pathway
- Intracellular Kinases
- Intracellular Kinases in the Akt Pathway
- Intracellular Signaling Molecules in Angiogenesis
- Multiple Myeloma
- Neurotrophin/Trk Family
- Notch Pathway
- PDGF Family
- Signaling Molecules and Transcriptional Regulators of Tfh Cell Differentiation
- VEGF Family
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参考图片Detection of Phospho-Akt (S473) in Jurkat Human Cell Line by Flow Cytometry. Jurkat human acute T cell leukemia cell line either untreated (open histogram) or treated with 100 nM Calyculin for 30 minutes (filled histogram) was stained with Mouse Anti-Human/Mouse Phospho-Akt (S473) PE‑conjugated Monoclonal Antibody (Catalog # IC7794P). To facilitate intracellular staining, cells were fixed with Flow Cytometry Fixation Buffer (Catalog # FC004) and permeabilized with Flow Cytometry Permeabilization/Wash Buffer I (Catalog # FC005). View our protocol for Staining Intracellular Molecules. |
