Mouse TNF-alpha Phycoerythrin MAb (Clone MP6-XT22) (100 TESTS)
货号：
IC410P 基本售价：
4382.2 元 规格：
100 Tests
产品信息
概述| 货号 | IC410P |
| 别名 | APC1 protein; Cachectin; Cachetin; DIF; TNF; TNF, monocyte-derived; tnfa; tnf-a; TNF-alphacachectin; TNFATNF, macrophage-derived; TNFSF1A; TNFSF2; TNFSF2TNF superfamily, member 2; tumor necrosis factor (TNF superfamily, member 2); tumor necrosis factor alpha; Tumor necrosis factor ligand superfamily member 2; tumor necrosis factor; tumor necrosis factor-alpha |
全称 | Tumor Necrosis Factor alpha | | 反应种属 | Mouse |
| 应用 | Intracellular Staining by Flow Cytometry(10 µL/106cells)
|
| 目标/特异性 | Detects mouse TNF-alpha in flow cytometry. |
| 使用方法 | Intracellular Staining by Flow Cytometry: 10 µL/106cells
|
| 来源 | The product is shipped with polar packs. Upon receipt, store it immediately at the temperature recommended below. |
| 产品组分 | |
性能| 供应商 | R&D Systems |
| Entrez Gene IDs | 7124 (Human); 21926 (Mouse); 24835 (Rat); 397086 (Porcine); 280943 (Bovine); 403922 (Canine); 100033834 (Equine); 493755 (Feline); 100009088 (Rabbit) |
| 纯化方式 | Protein A or G purified from hybridoma culture supernatant |
| 免疫原 | Mouse TNF-alpha |
| 生物活性 | Mouse |
| 标记 | Phycoerythrin |
| 背景 | TNF-alpha is a trimeric glycoprotein active in both membrane bound and secreted forms. TNF-alpha is produced by several lymphoid cells as well as by astrocytes, endothelial cells, and smooth muscle cells. TNF-alpha binds to TNF RI and TNF RII present on virtually all cell types, through which it triggers the activation of multiple signal transduction pathways and modulates the expression of a wide variety of genes. Over the mature regions, human and mouse share 66% amino acid sequence identity. |
| 运输条件 | Blue Ice |
| 存放说明 | 4℃ |
| 参考文献 | - Adipocytokines
- Cancer Biomarkers
- Conventional/Classical Dendritic Cells
- Co-stimulatory and Co-inhibitory Molecules
- Cytokines and Receptors in Allergy and Asthma
- Cytokines and Receptors in Angiogenesis
- Cytokines in Lipid/Cholesterol Metabolism
- Diabetes
- Diabetic Peripheral Neuropathy
- EMT Induction
- gamma delta T Cells
- ICC / IHC Images: TNF-alpha
- Inflammatory Mediators
- Inflammatory/Monocyte-derived Dendritic Cells
- Interactive Pathway: Adipocytokines & Insulin Signaling
- Isolation, Culture, and Identification of Natural Killer Cells
- Macrophage Activation Markers
- Natural Killer T (NKT) Cells
- Neuroinflammation
- Obesity
- Plasmacytoid Dendritic Cells
- Preeclampsia
- Regulation of Apoptosis by TNF Superfamily Members
- Regulation of Natural Killer Cell Co-stimulation by TNF Superfamily Members
- Regulation of T Cell Co-stimulation by TNF Superfamily Members
- T Cell Cytokine Signaling
- Technical Notes: R&D Systems Is With You Every Step of the Way - Analyzing Proteins of Interest
- Th1 Cells
- Th22 Cells
- TNF Superfamily Ligands
|
参考图片Detection of TNF‑ alpha in EL‑4 Mouse Cell Line by Flow Cytometry. EL‑4 mouse lymphoblast cell line treated with anti-CD3/anti-CD28, PMA, and Calcium Ionomycin was stained with Rat Anti-Mouse TNF‑ alpha PE‑conjugated Monoclonal Antibody (Catalog # IC410P, filled histogram) or isotype control antibody (Catalog # IC005P, open histogram). To facilitate intracellular staining, cells were fixed with Flow Cytometry Fixation Buffer (Catalog # FC004) and permeabilized with Flow Cytometry Permeabilization/Wash Buffer I (Catalog # FC005). View our protocol for Staining Intracellular Molecules. |
