Mouse IL-7 R alpha/CD127 Allophycocyanin MAb (Clone A7R34) (100 TESTS)【科瑞奥博】

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Mouse IL-7 R alpha/CD127 Allophycocyanin MAb (Clone A7R34) (100 TESTS)

货号： FAB47742A 基本售价： 4382.2 元规格： 100 Tests

产品信息

概述
货号	FAB47742A
别名	CD127 antigen; CD127; CD127ILRA; CDW127; IL-7 receptor subunit alpha; IL-7R subunit alpha; IL7R; IL7RA; IL-7RA; IL-7R-alpha; interleukin 7 receptor alpha chain; interleukin 7 receptor isoform H5-6; interleukin 7 receptor; interleukin-7 receptor subunit alpha
全称	Interleukin 7 Receptor alpha
反应种属	Mouse
应用	Flow Cytometry(10 µL/10⁶cells)
目标/特异性	Detects mouse IL‑7 R alpha /CD127 in flow cytometry.
使用方法	Flow Cytometry: 10 µL/10⁶cells
来源	The product is shipped with polar packs. Upon receipt, store it immediately at the temperature recommended below.
产品组分

性能
供应商	R&D Systems
Entrez Gene IDs	3575 (Human); 16197 (Mouse); 294797 (Rat)
纯化方式	Protein A or G purified from hybridoma culture supernatant
免疫原	Recombinant mouse IL‑7 R alpha /CD127
生物活性	Mouse
标记	Allophycocyanin
背景	Interleukin 7 Receptor alpha (IL-7 R alpha ), also known as CD127, is a 75 kDa hematopoietin receptor superfamily member that plays an important role in lymphocyte differentiation, proliferation, and survival (1, 2). Mature mouse IL-7 R alpha consists of a 219 amino acid (aa) extracellular domain (ECD) with one fibronectin type III domain and a WSxWS motif, a 25 aa transmembrane segment, and a 195 aa cytoplasmic domain (3). Within the ECD, mouse IL-7 R alpha shares 67% and 79% aa sequence identity with human and rat IL-7 R alpha, respectively. IL-7 R alpha associates with the common gamma chain ( gamma _c) to form the functional high affinity IL-7 receptor complex (4). The gamma _c is also a subunit of the receptors for IL-2, -4, -9, -15, and -21. Human and mouse IL-7 show cross-species activity through the IL-7 receptor (3, 5). IL-7 R alpha is expressed on double negative (CD4^-CD8^-) and CD4⁺ or CD8⁺ single positive T cells as well as on CD8⁺ memory T cells and their precursors (6, 7). It is expressed early in B cell development, prior to the appearance of surface IgM (6). In mice, IL-7 activation of IL-7 R alpha is critical for both T cell and B cell lineage development (8). In humans it is required for T cell but not for B cell development (9). IL-7 induces the down regulation and shedding of cell surface IL-7 R alpha (10). IL-7 R alpha additionally associates with TSLP R to form the functional receptor for thymic stromal lymphopoietin (11, 12). TSLP indirectly regulates T cell development by modulating dendritic cell activation (2, 13). Knockout of TSLP R in mice provokes minor changes in B and T cell development compared to those seen with IL-7 R alpha deletion (8, 14). The complexity of IL-7 R alpha biology is suggested by the competition between IL-7 and TSLP for receptor binding and by the ability of IL-7 R alpha to form functional complexes with SCF R and HGF R (11, 12, 15, 16).
运输条件	Blue Ice
存放说明	4℃
参考文献	Mazzucchelli, R. and S.K. Durum, 2007, Nat. Rev. Immunol. 7:144. Liu, Y.-J. et al. (2007) Annu. Rev. Immunol. 25:193. Goodwin, R.G. et al. (1990) Cell 60:941. Noguchi, M. et al. (1993) Science 262:1877. Barata, J.T. et al. (2006), Exp. Hematol. 34:1133. Sudo, T. et al. (1993) Proc. Natl. Acad. Sci. 90:9125. Kaech, S.M. et al. (2003) Nat. Immunol. 4:1191. Peschon, J.J. et al. (1994) J. Exp. Med. 180:1955. Prieyl, J.A. and T.W. LeBien (1996) Proc. Natl. Acad. Sci. 93:10348. Vranjkovic, A. et al. (2007) Int. Immunol. 19:1329. Park, L.S. et al. (2000) J. Exp. Med. 192:659. Pandey, A. et al. (2000) Nat. Immunol. 1:59. Reche, P.A. et al. (2001) J. Immunol. 167:336. Al-Shami, A. et al. (2004) J. Exp. Med. 200:159. Jahn, T. et al. (2007) Blood 110:1840. Lai, L. et al. (2006) Blood 107:1776.

参考图片

Detection of IL‑7 R alpha /CD127 in Mouse Splenocytes by Flow Cytometry. Mouse splenocytes were stained with Rat Anti-Mouse CD3 PE‑conjugated Monoclonal Antibody (Catalog # FAB4841P) and either (A) Rat Anti-Mouse IL‑7 R alpha /CD127 APC‑conjugated Monoclonal Antibody (Catalog # FAB47742A) or (B) Rat IgG_2A Allophycocyanin Isotype Control (Catalog # IC006A). View our protocol for Staining Membrane-associated Proteins.