| 货号 | IC8224G |
| 别名 | FLJ36364; MGC47735; T-cell factor 1; T-cell specific; TCF1; TCF-7; transcription factor 7 (T-cell specific, HMG-box) | 全称 | Transcription Factor 7 |
| 反应种属 | Mouse |
| 应用 | Intracellular Staining by Flow Cytometry(5 µL/106cells) |
| 目标/特异性 | Detects mouse TCF7/TCF1 in direct ELISAs and Western Blots. |
| 使用方法 | Intracellular Staining by Flow Cytometry: 5 µL/106cells |
| 来源 | The product is shipped with polar packs. Upon receipt, store it immediately at the temperature recommended below. |
| 产品组分 |
| 供应商 | R&D Systems |
| Entrez Gene IDs | 6932 (Human); 21414 (Mouse); 363595 (Rat) |
| 纯化方式 | Protein A or G purified from hybridoma culture supernatant |
| 免疫原 | E. coli-derived recombinant mouse TCF7/TCF1 Gly61-Gly116 Accession # Q00417 |
| 生物活性 | Mouse |
| 标记 | Alexa Fluor 488 |
| 背景 | TCF7 (Transcription Factor 7), also known as TCF1 (T cell Factor 1) is a member of the Lymphoid Enhancer Binding Factor family of proteins that is expressed in thymocytes and mature T cells. In resting cells, TCF family members are transcriptional repressors, and are 25‑32 kDa in size. Following activation, the large TCF7/TCF1 isoform (isoform 2, chosen as canonical) predominates (419 amino acids, 42‑50 kDa), and serves a transcriptional activator function. The use of an alternate start site at Met116 seems to characterize repressor isoforms. Within amino acids (aa) 61-116 of the large form, mouse TCF7/TCF1 shares 77% aa sequence identity with human TCF7/TCF1. |
| 运输条件 | Blue Ice |
| 存放说明 | 4℃ |
| 参考文献 |
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Detection of TCF7/TCF1 in EL‑4 Mouse Cell Line by Flow Cytometry. EL‑4 mouse lymphoblast cell line was stained with Rat Anti-Mouse TCF7/TCF1 Alexa Fluor® 488‑conjugated Monoclonal Antibody (Catalog # IC8224G, filled histogram) or isotype control antibody (Catalog # IC013G, open histogram). To facilitate intracellular staining, cells were fixed with Flow Cytometry Fixation Buffer (Catalog # FC004) and permeabilized with Flow Cytometry Permeabilization/Wash Buffer I (Catalog # FC005). View our protocol for Staining Intracellular Molecules. |
