Human/Mouse IL-12/IL-35 p35 Phycoerythrin MAb (Clone 27537) (100 TESTS)【科瑞奥博】

产品中心

当前位置：首页>产品中心

Human/Mouse IL-12/IL-35 p35 Phycoerythrin MAb (Clone 27537) (100 TESTS)

货号： IC2191P 基本售价： 4382.2 元规格： 100 Tests

产品信息

概述
货号	IC2191P
别名	IL-12A
全称	Interleukin 12 p35
反应种属	Human/Mouse
应用	Intracellular Staining by Flow Cytometry(10 µL/10⁶cells)
目标/特异性	Detects human IL‑12/IL-35 p35 in direct ELISAs and Western blots. Detects the p35 subunit either as part of a p40/p35 heterodimer or as a free subunit after reduction of the heterodimer. This antibody does not recognize IL-12 p40 homodimers but shows strong cross-reactivity with the p35 subunits from porcine and mouse systems.
使用方法	Intracellular Staining by Flow Cytometry: 10 µL/10⁶cells
来源	The product is shipped with polar packs. Upon receipt, store it immediately at the temperature recommended below.
产品组分

性能
供应商	R&D Systems
Entrez Gene IDs	3592 (Human); 16159 (Mouse); 84405 (Rat)
应用文献
R&D Systems personnel manually curate a database that contains references using R&D Systems products. The data collected includes not only links to publications in PubMed, but also provides information about sample types, species, and experimental conditions. IL-35 over-expression increases apoptosis sensitivity and suppresses cell growth in human cancer cells. Biochem Biophys Res Commun, 2013;430(1):364-9. Species: Human Sample Type: whole cells Application: Flow Generation of inducible immortalized dendritic cells with proper immune function in vitro and in vivo. Authors: Richter C, Thieme S, Bandola J, Laugsch M, Anastassiadis K, Brenner S PLoS ONE, 2013;8(4):e62621. Species: Mouse Sample Type: Whole Cells Application: Flow Cutting edge: Human regulatory T cells require IL-35 to mediate suppression and infectious tolerance. Authors: Chaturvedi V, Collison LW, Guy CS, Workman CJ, Vignali DA J. Immunol., 2011;186(12):6661-6. Species: Human Sample Type: Whole Cells Application: Flow
纯化方式	Protein A or G purified from ascites
免疫原	S. frugiperda insect ovarian cell line Sf 21-derived recombinant human IL‑12/IL-35 p35 Arg23-Ser219 Accession # P29459
生物活性	Human, Mouse
标记	Phycoerythrin
背景	Interleukin12 (IL-12) and interleukin 35 (IL-35) are heterodimeric cytokines composed of alpha and beta chains. IL-12 is composed of p35 and p40 subunits, while IL-35 iscomprised of p35 paired with EBI-3 (1). In mice, IL-35 is produced by FoxP3⁺regulatory T cells and may function as an inhibitory cytokine to suppress Tcell proliferation (2). Human FoxP3⁺ Tregs do not constitutivelyexpress IL-35 (3), but expression may be induced by activated dendritic cells(4).
运输条件	Blue Ice
存放说明	4℃
参考文献	Collison, L.W. and D.A.A. Vignali (2008) Immunol. Rev. 226:248. Collison, L.W. et al. (2007) Nature 450:566. Bardel,E.et al. (2008) J. Immunol. 181:6898. Seyerl, M. et al.(2010) Eur. J. Immunol. 40:321.

参考图片

Detection of IL‑12/IL‑35 p35 in Human PBMCs by Flow Cytometry. Human peripheral blood mononuclear cells (PBMCs) either untreated (orange open histogram) or treated with LPS (orange filled histogram) were stained with Mouse Anti-Human/Mouse IL‑12/IL‑35 p35 PE‑conjugated Monoclonal Antibody (Catalog # IC2191P) or isotype control antibody (Catalog # IC002P, blue open histogram). To facilitate intracellular staining, cells were fixed with Flow Cytometry Fixation Buffer (Catalog # FC004) and permeabilized with Flow Cytometry Permeabilization/Wash Buffer I (Catalog # FC005). View our protocol for Staining Intracellular Molecules.

Detection of IL‑12/IL‑35 p35 in Mouse Splenocytes by Flow Cytometry. Mouse splenocytes either untreated (orange open histogram) or treated with LPS (orange filled histogram) were stained with Mouse Anti-Human/Mouse IL‑12/IL‑35 p35 PE‑conjugated Monoclonal Antibody (Catalog # IC2191P) or isotype control antibody (Catalog # IC002P, blue open histogram). To facilitate intracellular staining, cells were fixed with Flow Cytometry Fixation Buffer (Catalog # FC004) and permeabilized with Flow Cytometry Permeabilization/Wash Buffer I (Catalog # FC005). View our protocol for Staining Intracellular Molecules.