Human/Mouse Oct-3/4 Phycoerythrin MAb (Clone 240408) (100 TESTS)【科瑞奥博】

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Human/Mouse Oct-3/4 Phycoerythrin MAb (Clone 240408) (100 TESTS)

货号： IC1759P-100 基本售价： 4382.2 元规格： 100 Tests

产品信息

概述
货号	IC1759P-100
别名	Otf3g; Otf4; Pou5f1
全称	Octamer-binding Protein 3/4
反应种属	Human/Mouse
应用	Intracellular Staining by Flow Cytometry(10 µL/10⁶cells)
目标/特异性	Detects human Oct-3/4 in Western blots and detects mouse Oct-3/4 in flow cytometry.
使用方法	Intracellular Staining by Flow Cytometry: 10 µL/10⁶cells
来源	The product is shipped with polar packs. Upon receipt, store it immediately at the temperature recommended below.
产品组分

性能
供应商	R&D Systems
Entrez Gene IDs	5460 (Human); 18999 (Mouse)
应用文献
R&D Systems personnel manually curate a database that contains references using R&D Systems products. The data collected includes not only links to publications in PubMed, but also provides information about sample types, species, and experimental conditions. Phenotypic diversity of patient-derived melanoma populations in stem cell medium. Authors: Sztiller-Sikorska M, Hartman M, Talar B, Jakubowska J, Zalesna I, Czyz M Lab Invest, 2015;95(6):672-83. Species: Human Sample Type: Whole Cells Application: Flow Rapid target gene validation in complex cancer mouse models using re-derived embryonic stem cells. Authors: Huijbers I, Bin Ali R, Pritchard C, Cozijnsen M, Kwon M, Proost N, Song J, de Vries H, Badhai J, Sutherland K, Krimpenfort P, Michalak E, Jonkers J, Berns A EMBO Mol Med, 2014;6(2):212-25. Species: Mouse Sample Type: Whole Cells Application: Flow Cross-presentation of tumour antigens by human induced pluripotent stem cell-derived CD141(+)XCR1+ dendritic cells. Gene Ther, 2012;19(10):1035-40. Species: Human Sample Type: Whole Cells Application: Flow Human embryonic stem cells with maintenance under a feeder-free and recombinant cytokine-free condition. Authors: Nakahara M, Saeki K, Nakamura N Cloning Stem Cells, 2009;11(1):5-18. Species: Human Sample Type: Whole Cells Application: Flow Octamer 4 small interfering RNA results in cancer stem cell-like cell apoptosis. Authors: Hu T, Liu S, Breiter DR, Wang F, Tang Y, Sun S Cancer Res., 2008;68(16):6533-40. Species: Human Sample Type: Whole Cells Application: Flow Kidney tissue reconstruction by fetal kidney cell transplantation: effect of gestation stage of fetal kidney cells. Authors: Kim SS, Gwak SJ, Han J, Park HJ, Park MH, Song KW, Cho SW, Rhee YH, Chung HM, Kim BS Stem Cells, 2007;25(6):1393-401. Species: Rat Sample Type: Whole Cells Application: Flow
纯化方式	Protein A or G purified from hybridoma culture supernatant
免疫原	E. coli-derived recombinant human Oct‑3/4 Met1-Asn265 (Met262Leu) Accession # Q01860
生物活性	Human, Mouse
标记	Phycoerythrin
背景	Oct-3/4, also termed Oct-3 or Oct-4, is a POU transcription factor that is expressed in totipotent embryonic stem and germ cells (1- 3). Oct-3/4 is required to sustain stem cell self-renewal and pluripotency (4). It is considered a master regulator of pluripotency that controls lineage commitment and is the most widely recognized marker of totipotent embryonic stem cells.
运输条件	Blue Ice
存放说明	4℃
参考文献	Takeda, J. et al. (1992) Nucleic Acids Res. 20:4613. Scholer, H.R. et al. (1989) EMBO J. 8:2543. Rosner, M.H. et al. (1990) Nature 345:686. Niwa, H. et al. (2000) Nat. Genet. 24:372.

参考图片

Detection of Oct‑3/4 in BG01V Human Cells by Flow Cytometry. BG01V human embryonic stem cells were stained with Rat Anti-Human/Mouse Oct‑3/4 PE‑conjugated Monoclonal Antibody (Catalog # IC1759P, filled histogram) or isotype control antibody (Catalog # IC013P, open histogram). To facilitate intracellular staining, cells were fixed with Flow Cytometry Fixation Buffer (Catalog # FC004) and permeabilized with Flow Cytometry Permeabilization/Wash Buffer I (Catalog # FC005). View our protocol for Staining Intracellular Molecules.

Detection of Oct‑3/4 in Mouse Stem Cells by Flow Cytometry. Mouse embryonic stem cells treated with 5 μM Retinoic Acid for 3 days were stained with Rat Anti-Human/Mouse Oct‑3/4 PE‑conjugated Monoclonal Antibody (Catalog # IC1759P, filled histogram) or isotype control antibody (Catalog # IC013P, open histogram). To facilitate intracellular staining, cells were fixed with Flow Cytometry Fixation Buffer (Catalog # FC004) and permeabilized with Flow Cytometry Permeabilization/Wash Buffer I (Catalog # FC005). View our protocol for Staining Intracellular Molecules.