Mouse TER-119 Phycoerythrin MAb (Clone TER-119) (100 TESTS)
货号：
FAB1125P 基本售价：
4243.4 元 规格：
100 Tests
产品信息
概述| 货号 | FAB1125P |
| 别名 | Ly76; Ly-76; TER119 |
| 反应种属 | Mouse |
| 应用 | Flow Cytometry(10 µL/106cells)
|
| 目标/特异性 | Detects mouse TER‑119 in Western blots. This antibody has been shown to react with cells of the erythroid lineage in embryonic yolk sac, fetal liver, adult bone marrow, adult peripheral blood, and adult lymphoid organs (Ikuta, K. et al. (1990) Cell 62:863). TER-119 Antigen is a molecule associated with cell-surface glycophorin A (Kina, T. et al. (2000) Br. J. Haematol. 109:280). The antibody has been shown to react with erythroid cells at differentiation stages from early proerythroblast to mature erythrocyte (Kina, T. et al. (2000) Br. J. Haematol. 109:280). |
| 使用方法 | Flow Cytometry: 10 µL/106cells
|
| 来源 | The product is shipped with polar packs. Upon receipt, store it immediately at the temperature recommended below. |
| 产品组分 | |
性能| 供应商 | R&D Systems |
| Entrez Gene IDs | 104231 (Mouse) |
| 应用文献 |
| R&D Systems personnel manually curate a database that contains references using R&D Systems products. The data collected includes not only links to publications in PubMed, but also provides information about sample types, species, and experimental conditions. Infection with Anaplasma phagocytophilum induces multilineage alterations in hematopoietic progenitor cells and peripheral blood cells.
Authors: Johns JL, Macnamara KC, Walker NJ, Winslow GM, Borjesson DL
Infect. Immun., 2009;77(9):4070-80.
Species: Mouse
Sample Type: Whole Cells
Application: Flow |
| 纯化方式 | Protein A or G purified from hybridoma culture supernatant |
| 免疫原 | C57BL/6 mouse day-14 fetal liver cells |
| 生物活性 | Mouse |
| 标记 | Phycoerythrin |
| 背景 | The monoclonal antibody TER-119 is isolated from a hybridoma generated using splenocytes from a rat subcutaneously injected with day 14 BALB/c fetal liver cells (1). The TER-119 monoclonal antibody reacts with erythroid cells from the early proerythroblast to mature erythrocyte stages of development (1). The 52 kDa ligand for TER-119 is associated with glycophorin A on erythrocytes (1). TER-119 antibodies are frequently used in combination with other lineage depletion antibodies to enrich for mouse hematopoietic stem cells (2, 3). |
| 运输条件 | Blue Ice |
| 存放说明 | 4℃ |
| 参考文献 | - Kina, T. et al. (2000) Br. J. Haematol. 109:280.
- Ikuta, K. et al. (1990) Cell 62:863.
- Osawa, M.Y. et al., (1996) Hematopoietic Stem Cells in Weir’s Handbook of Experimental Immunology, Vol. 2, 5th Edition. Herzenberg, L.A. et al. eds. Blackwell Science, Cambridge, MA. pp. 66.1-66.5
|
参考图片Detection of TER‑119 in Balb/c Mouse Bone Marrow Cells by Flow Cytometry. Balb/c mouse bone marrow cells was stained with Rat Anti-Mouse TER‑119 PE‑conjugated Monoclonal Antibody (Catalog # FAB1125P) and Rat Anti-Mouse B220/CD45R Fluorescein‑conjugated Monoclonal Antibody (Catalog # FAB1217F). Quadrant markers were set based on control antibody staining. View our protocol for Staining Membrane-associated Proteins. |
