Mouse B220/CD45R Alexa Fluor 488 MAb (Clone RA3-6B2) (100 TESTS)【科瑞奥博】

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Mouse B220/CD45R Alexa Fluor 488 MAb (Clone RA3-6B2) (100 TESTS)

货号： FAB1217G 基本售价： 4243.4 元规格： 100 Tests

产品信息

概述
货号	FAB1217G
别名	B220; CD45 antigen; CD45R; EC 3.1.3.48; L-CA; LY5; protein tyrosine phosphatase, receptor type, C; receptor-type tyrosine-protein phosphatase C; T200 glycoprotein; T200 leukocyte common antigen; T200receptor type, c polypeptide
全称	Cluster of Differentiation 45
反应种属	Mouse
应用	Flow Cytometry(10 µL/10⁶cells) Immunocytochemistry(8-25 µg/mL)
目标/特异性	Detects mouse B220/CD45R.
使用方法	Flow Cytometry: 10 µL/10⁶cells Immunocytochemistry: 8-25 µg/mL
来源	The product is shipped with polar packs. Upon receipt, store it immediately at the temperature recommended below.
产品组分

性能
供应商	R&D Systems
Entrez Gene IDs	5788 (Human); 19264 (Mouse); 24699 (Rat)
纯化方式	Protein A or G purified from hybridoma culture supernatant
免疫原	Abelson mouse leukemia virus-induced pre-B tumor cells
生物活性	Mouse
标记	Alexa Fluor 488
背景	B220 (accession # P06800) is the mouse CD45 isoform CD45R, which is predominantly expressed on all B lymphocytes, including pro-, mature and activated B cells (1‑3). The level of B220 antigen expression on the B cell lineage is developmentally regulated (2‑4) and this antibody is commonly used as a B cell marker. RA3-6B2 has been reported to inhibit in vivo B cell responses (5, 6).
运输条件	Blue Ice
存放说明	4℃
参考文献	Coffman, R.L. (1982) Immuno. Rev. 69:5. Hardy, R.R. et al. (1991) J. Exp. Med. 173:1213. Hathcock, K.S. et al. (1992) J. Immunol. 149:2286. Allman, D.M. et al. (1992) J. Immunol. 149:2533. Asensi, V.K. et al. (1989) Immunology 68:204. Domiati-Saad, R. et al. (1993) J. Immunol. 151:5936.

参考图片

Detection of B220/CD45R in Mouse Splenocytes by Flow Cytometry. Mouse splenocytes were stained with Rat Anti-Mouse CD3 APC‑conjugated Monoclonal Antibody (Catalog # FAB4841A) and either (A) Rat Anti-Mouse B220/CD45R Alexa Fluor® 488‑conjugated Monoclonal Antibody (Catalog # FAB1217G) or (B) Rat IgG_2A Alexa Fluor 488 Isotype Control (Catalog # IC006G). View our protocol for Staining Membrane-associated Proteins.

B220/CD45R in Mouse Splenocytes. B220/CD45R was detected in immersion fixed mouse splenocytes using Rat Anti-Mouse B220/CD45R Alexa Fluor® 488‑conjugated Monoclonal Antibody (Catalog # FAB1217G; green) at 5 µg/mL for 3 hours at room temperature. Cells were counterstained with DAPI (blue). View our protocol for Fluorescent ICC Staining of Non-adherent Cells.