| 货号 | FAB16861N-100 |
| 别名 | IBD17; IL-23 receptor; IL-23R; interleukin 23 receptor; interleukin-23 receptor | 全称 | Interleukin 23 Receptor |
| 反应种属 | Mouse |
| 应用 | Flow Cytometry(5 µL/106cells) |
| 目标/特异性 | Detects endogenous mouse IL-23 R by flow cytometry. |
| 使用方法 | Flow Cytometry: 5 µL/106cells |
| 来源 | The product is shipped with polar packs. Upon receipt, store it immediately at the temperature recommended below. |
| 产品组分 |
| 供应商 | R&D Systems |
| Entrez Gene IDs | 149233 (Human); 209590 (Mouse) |
| 应用文献 | |
| R&D Systems personnel manually curate a database that contains references using R&D Systems products. The data collected includes not only links to publications in PubMed, but also provides information about sample types, species, and experimental conditions. IL-17-producing gammadelta T cells and neutrophils conspire to promote breast cancer metastasis. | |
| 纯化方式 | Protein A or G purified from hybridoma culture supernatant |
| 免疫原 | Mouse myeloma cell line NS0-derived recombinant mouse IL-23 R Gly24-Asp372 Accession # Q8K4B4 |
| 生物活性 | Mouse |
| 标记 | Alexa Fluor 700 |
| 背景 | Interleukin 23 (IL-23) is a heterodimeric cytokine composed of two disulfide-linked subunits, a p19 subunit that is unique to IL-23, and a p40 subunit that is shared with IL-12 (1‑5). The functional IL-23 receptor complex consists of two receptor subunits, the IL-12 receptor beta 1 subunit (IL-12 R beta 1) and the IL-23-specific receptor subunit (IL-23 R) (3). Mouse IL-23 R cDNA encodes a 644 amino acid (aa) type I transmembrane protein with a 23 aa residue signal peptide, a 349 aa residue extracellular domain, a 23 aa residue transmembrane domain and a 249 aa residue cytoplasmic region. IL-23 R shares structural features with the IL-12 R beta 2, including an N-terminal Ig-like domain, two cytokine receptor domains and multiple glycosylation sites in the extracellular domain. IL-23 R lacks the three extracellular membrane-proximal fibronectin-type III domains present on IL-12 R beta 2. IL-23 R has a WQPWS sequence in the transmembrane-proximal cytokine receptor domain similar to the cytokine receptor signature WSXWS motif. The cytoplasmic region of IL-23 R has three potential Src homology 2 domain-binding sites and two potential Stat-binding sites. The gene for human IL-23 R is located on human chromosome 1 within 150 kb of IL-12 R beta 2. Human and mouse IL-23 R share 66% amino acid sequence identity. Mouse IL-23 R is expressed in mouse Th1 and Th2 cells, bone marrow, dendritic cells and macrophages. It is also expressed by mouse CD4+CD45RBlow memory T cells but at much lower levels by mouse CD4+CD45RBhigh cells. IL-23 initiates a signal transduction cascade similar to that of IL-12 and involves Jak2, Tyk2, STAT1, STAT3, STAT4, and STAT5. IL-23 has biological activities that are similar to, but distinct from, IL-12. |
| 运输条件 | Blue Ice |
| 存放说明 | 4℃ |
| 参考文献 |
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Detection of IL‑23 R in Mouse Splenocytes by Flow Cytometry. Mouse splenocytes treated with 10 μg/mL Anti-CD3 (Catalog #MAB484),5 μg/mL Anti-CD28 (Catalog # AF483),10 ng/mL Recombinant Mouse TGF-beta 1 (Catalog # 100-B),20 ng/mL Recombinant Mouse IL-23 (Catalog # 1887-ML),40 ng/mL Recombinant Mouse IL-6 (Catalog # 406-ML),and 10 ng/mL Recombinant Mouse IL-1 beta (Catalog # 401-ML)for 5 days to induce T17 activation were stained with Rat Anti-Mouse CD4 Fluorescein‑conjugated MonoclonalAntibody (Catalog # FAB554F) and either (A) Rat Anti-Mouse IL‑23 RAlexa Fluor® 700‑conjugated Monoclonal Antibody (Catalog # FAB16861N)or (B) Rat IgG1 Alexa Fluor 700 Isotype Control(Catalog # IC005N).View our protocol for StainingMembrane-associated Proteins. | |
Detection of IL‑23 R in HEK293 Human Cell Line Transfected with Mouse IL-23 R by Flow Cytometry. HEK293 human embryonic kidney cell line transfected with (A) mouse IL-23 R or (B) irrelevant transfectants was stained with Rat Anti-Mouse IL‑23 R Alexa Fluor® 700‑conjugated Monoclonal Antibody (Catalog # FAB16861N, filled histogram) or isotype control antibody (Catalog # IC005N, open histogram). View our protocol for Staining Membrane-associated Proteins. |
