| 货号 | FAB168P |
| 别名 | Activation-inducible lymphocyte immunomediatory molecule; AILIM; AILIMCD278; CD278 antigen; CD278; CRP-1; CVID1; inducible costimulator; inducible T-cell costimulator; inducible T-cell co-stimulator; MGC39850 | 全称 | Inducible Co-stimulator |
| 反应种属 | Mouse |
| 应用 | Flow Cytometry(10 µL/106cells) |
| 目标/特异性 | Detects mouse ICOS in direct ELISAs. |
| 使用方法 | Flow Cytometry: 10 µL/106cells |
| 来源 | The product is shipped with polar packs. Upon receipt, store it immediately at the temperature recommended below. |
| 产品组分 |
| 供应商 | R&D Systems |
| Entrez Gene IDs | 29851 (Human); 54167 (Mouse) |
| 纯化方式 | Protein A or G purified from hybridoma culture supernatant |
| 免疫原 | NS0 mouse myeloma cell line transfected with mouse ICOS Accession # Q9WVS0 |
| 生物活性 | Mouse |
| 标记 | Phycoerythrin |
| 背景 | Mouse ICOS (Inducible Co-Stimulator), also known as AILIM (Activiation-Inducible Lymphocyte Immunomediatory Molecule) and CRP-1 (CD28-Related Protein-1), is a member of the CD28 family of immune costimulatory receptors. Other family members are CD28, CTLA-4 and PD-1. Mouse ICOS is a homodimeric type I transmembrane protein consisting of 200 amino acids (aa) with a putative 20 aa signal sequence, a 122 aa extracellular domain, a 23 aa transmembrane region, and a 35 aa cytoplasmic domain. ICOS shares approximately 39% aa similarity with CD28 and CTLA-4. Mouse and human ICOS share approximately 72% aa identity. ICOS is expressed on most CD45RO+ cells. ICOS expression is upregulated within approximately 24-48 hours of activation on Th primed cells. B7-H2, a member of the B7 family of costimulatory ligands, has been identified as the ICOS ligand. The B7-H2/ICOS interaction appears to play roles in T cell dependent B cell activation and Thdifferentiation. |
| 运输条件 | Blue Ice |
| 存放说明 | 4℃ |
| 参考文献 |
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Detection of ICOS in Mouse Splenocytes by Flow Cytometry. Mouse splenocytes treated with 1 μg/mL Concanavalin A for 3 days were stained with Rat Anti-Mouse ICOS PE‑conjugated Monoclonal Antibody (Catalog # FAB168P, filled histogram) or isotype control antibody (Catalog # IC013P, open histogram). View our protocol for Staining Membrane-associated Proteins. |
