| 货号 | FAB6524P |
| 别名 | CO-029; tetraspanin 8; TM4SF3; TM4SF3tspan-8; Transmembrane 4 superfamily member 3tetraspanin-8; Tspan-8; Tumor-associated antigen CO-029 | 全称 | Tetraspanin 8/Transmembrane 4 Superfamily, Member 3 |
| 反应种属 | Mouse |
| 应用 | Flow Cytometry(10 µL/106cells) |
| 目标/特异性 | Detects mouse TSPAN8 in direct ELISAs. |
| 使用方法 | Flow Cytometry: 10 µL/106cells |
| 来源 | The product is shipped with polar packs. Upon receipt, store it immediately at the temperature recommended below. |
| 产品组分 |
| 供应商 | R&D Systems |
| Entrez Gene IDs | 7103 (Human); 216350 (Mouse); 171048 (Rat) |
| 应用文献 | |
| R&D Systems personnel manually curate a database that contains references using R&D Systems products. The data collected includes not only links to publications in PubMed, but also provides information about sample types, species, and experimental conditions. Identification of quiescent and spatially restricted mammary stem cells that are hormone responsive | |
| 纯化方式 | Protein A or G purified from hybridoma culture supernatant |
| 免疫原 | CHO Chinese hamster ovary cell line transfected with mouse TSPAN8 Accession # Q8R3G9 |
| 生物活性 | Mouse |
| 标记 | Phycoerythrin |
| 背景 | TSPAN8(Tetraspanin-8), also known as TM4SF3 and CO-029, is a 27-33 kDa member of thetetraspanin family of proteins. Asevidenced by its name, it is a 4-transmembrane (glyco)protein that is foundon/in multiple tumor types as well as smooth and skeletal muscle, endotheliumand hematopoietic progenitor cells. All4-transmembrane proteins are not tetraspanins. Tetraspanins are characterized by the presence of a very short intracellularC- and N-terminus, and a very long extracellular (or LEL) domain linking the 3rdand 4th transmembrane segments. On TSPAN8 this domain is 98 amino acids (aa) in length and represents 42%of the entire length of mouse TSPAN8 (or 235 aa). TSPAN8, like other tetraspanins, forms thebackbone of a network that allows molecule interactions on (or in) plasmamembranes. This ability is dependentupon molecule palmitoylation and creates a microdomain (or TEM) that isdistinct from typical membrane rafts, the latter of which is characterized bythe presence of GPI-linked proteins and the molecule caveolin. Within this TEM, TSPAN8 may form homodimers,and is known to interact with multiple molecule types, including CD151, CD81,CD9, CD13, EpCAM, EWI-F, MMP-9, Meprin-beta, Rictor, TACE and the integrins alpha 3(CD49c) beta 1, alpha 6 beta 4 and alpha 4(CD49d) beta 1. Suchinteractions appear to positively or negatively modulate individual moleculeactivity. These interactions aregenerally described within the context of exosomes, 30-100 nm membrane-boundvesicles that are released from multiple cell types. TSPAN8-containing exosomes are reported toeither facilitate cell motility or act as an antiapoptotic agent for targetcells. Over aa 106-203 constituting theLEL, mouse TSPAN8 shares 58% and 73% aa sequence identity with human and ratTSPAN8, respectively. |
| 运输条件 | Blue Ice |
| 存放说明 | 4℃ |
| 参考文献 |
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Detection of TSPAN8 in CMT‑93 Mouse Cell Line by Flow Cytometry. CMT‑93 mouse rectal carcinoma cell line was stained with Rat Anti-Mouse TSPAN8 PE‑conjugated Monoclonal Antibody (Catalog # FAB6524P, filled histogram) or isotype control antibody (Catalog # IC013P, open histogram). View our protocol for Staining Membrane-associated Proteins. |
