Human/Mouse RelA/NFkB p65 Phycoerythrin MAb (Clone 532301) (100 TESTS)【科瑞奥博】

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Human/Mouse RelA/NFkB p65 Phycoerythrin MAb (Clone 532301) (100 TESTS)

货号： IC5078P 基本售价： 4243.4 元规格： 100 Tests

产品信息

概述
货号	IC5078P
别名	MGC131774; nf kb p65; NFkB p65; NF-kB p65; NFKB3; NFKB3v-rel avian reticuloendotheliosis viral oncogene homolog A (nuclear factor ofkappa light polypeptide gene enhancer in B-cells 3 (p65)); Nuclear factor NF-kappa-B p65 subunit; Nuclear factor of kappa light polypeptide gene enhancer in B-cells 3transcription factor p65; p65; p65RelA; rela p65; RelA; v-rel reticuloendotheliosis viral oncogene homolog A (avian); v-rel reticuloendotheliosis viral oncogene homolog A, nuclear factor of kappalight polypeptide gene enhancer in B-cells 3, p65
全称	v-rel Reticuloendotheliosis Viral Oncogene Homolog A
反应种属	Human/Mouse
应用	Intracellular Staining by Flow Cytometry(10 µL/10⁶cells)
目标/特异性	Detects human and mouse RelA/NF kappa B p65 in Western blots.
使用方法	Intracellular Staining by Flow Cytometry: 10 µL/10⁶cells
来源	The product is shipped with polar packs. Upon receipt, store it immediately at the temperature recommended below.
产品组分

性能
供应商	R&D Systems
Entrez Gene IDs	5970 (Human)
纯化方式	Protein A or G purified from hybridoma culture supernatant
免疫原	E. coli-derived recombinant human RelA/NF kappa B p65 isoform 1 Asn456-Ser551 Accession # Q04206
生物活性	Human, Mouse
标记	Phycoerythrin
背景	RelA p65 (v-rel reticuloendotheliosis viral oncogene homolog A) is a 65 kDa member of the NF kappa B family of nuclear transcription factors. Dimers of p65 with the p50 subunit are the most common form of the NF kappa B transcription factor, but dimers with it or other family members can also occur. Upon activation, RelA p65 forms an heterotetramer and moves into the nucleus where it binds to specific DNA sequences. An alternatively spliced isoform that lacks amino acids (aa) 222‑231 (p65 delta ) does not bind DNA. Over the sequence used as an immunogen, human RelA p65 shares 96% and 98% aa identity with mouse and rat RelA p65, respectively. This portion includes one of eight potential ser/thr phosphorylation sites, two acetylation sites, and most of the Rel homology domain that interacts with I kappa B inhibitors.
运输条件	Blue Ice
存放说明	4℃
参考文献	Apoptosis Transcription Factors and Regulators Chemokine Receptor Intracellular Signaling C-type Lectin Receptors ICC / IHC Images: RelA/NFkB p65 IL-1 Family IL-17 Family Immunoglobulin-Like Domain Transcription Factors Natural Killer T (NKT) Cells NFkB Pathway NOD-like Receptors and the Inflammasome PDGF Family RIG-I-like Receptors TNF Superfamily Regulators Toll-Like Receptors Transcription Factors and Regulators Transcription Factors in the Akt Pathway

参考图片

Detection of RelA/NF kappa B p65 in HeLa Human Cell Line by Flow Cytometry. HeLa human cervical epithelial carcinoma cell line was stained with Mouse Anti-Human/Mouse RelA/NF kappa B p65 PE‑conjugated Monoclonal Antibody (Catalog # IC5078P, filled histogram) or isotype control antibody (Catalog # IC0041P, open histogram). To facilitate intracellular staining, cells were fixed with Flow Cytometry Fixation Buffer (Catalog # FC004) and permeabilized with Flow Cytometry Permeabilization/Wash Buffer I (Catalog # FC005). View our protocol for Staining Intracellular Molecules.