货号 | IC2419P |
别名 | Glucose-sensitive factor; IDX1; IDX-1GSF; Insulin promoter factor 1; insulin promoter factor 1, homeodomain transcription factor; Insulin upstream factor 1; IPF1; IPF1pancreas/duodenum homeobox protein 1; Islet/duodenum homeobox-1; MODY4IUF1; pancreatic and duodenal homeobox 1; pancreatic-duodenal homeobox factor 1; PDX-1IPF-1; somatostatin transcription factor 1; Somatostatin-transactivating factor 1; STF-1IUF-1 | 全称 | Pancreas/Duodenum Homeobox-1 |
反应种属 | Human/Mouse |
应用 | Intracellular Staining by Flow Cytometry(10 µL/106cells) |
目标/特异性 | Detects human and mouse PDX-1/IPF1 in Western blots. |
使用方法 | Intracellular Staining by Flow Cytometry: 10 µL/106cells |
来源 | The product is shipped with polar packs. Upon receipt, store it immediately at the temperature recommended below. |
产品组分 |
供应商 | R&D Systems |
Entrez Gene IDs | 3651 (Human); 18609 (Mouse) |
应用文献 | |
R&D Systems personnel manually curate a database that contains references using R&D Systems products. The data collected includes not only links to publications in PubMed, but also provides information about sample types, species, and experimental conditions. Pancreatic islet cell phenotype and endocrine function throughout diabetes development in non-obese diabetic mice. | |
纯化方式 | Protein A or G purified from hybridoma culture supernatant |
免疫原 | E. coli-derived recombinant human PDX-1/IPF1 Ala91-Arg283 Accession # P52945 |
生物活性 | Human, Mouse |
标记 | Phycoerythrin |
背景 | Pancreatic-Duodenal Homeobox Factor-1 (PDX-1), also known as Insulin Promoter Factor 1 (IPF1), is a homeodomain transcription factor essential for pancreatic development and mature pancreatic beta cell function. |
运输条件 | Blue Ice |
存放说明 | 4℃ |
参考文献 |
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Detection of PDX‑1/IPF1 in beta TC‑6 Mouse Cell Line by Flow Cytometry. beta TC‑6 mouse beta cell insulinoma cell line was stained with Mouse Anti-Human/Mouse PDX‑1/IPF1 PE‑conjugated Monoclonal Antibody (Catalog # IC2419P, filled histogram) or isotype control antibody (Catalog # IC0041P, open histogram). To facilitate intracellular staining, cells were fixed with Flow Cytometry Fixation Buffer (Catalog # FC004) and permeabilized with Flow Cytometry Permeabilization/Wash Buffer I (Catalog # FC005). View our protocol for Staining Intracellular Molecules. | |
Detection of PDX‑1/IPF1 in BG01V Human Stem Cells Differentiated into Pancreatic Progenitor Cells by Flow Cytometry. BG01V human embryonic stem cells either (A) untreated or (B) differentiated into pancreatic progenitor cells were stained with Mouse Anti-Human/Mouse PDX‑1/IPF1 PE‑conjugated Monoclonal Antibody (Catalog # IC2419P, filled histogram) or isotype control antibody (Catalog # IC0041P, open histogram). To facilitate intracellular staining, cells were fixed with Flow Cytometry Fixation Buffer (Catalog # FC004) and permeabilized with Flow Cytometry Permeabilization/Wash Buffer I (Catalog # FC005). View our protocol for Staining Intracellular Molecules. |