| 货号 | FAB6758P |
| 别名 | Glycoprotein 6; glycoprotein VI (platelet); GP6; GPIV; GPVIplatelet collagen receptor; MGC138168; platelet glycoprotein VI | 全称 | Glycoprotein VI [Platelet] |
| 反应种属 | Mouse |
| 应用 | Flow Cytometry |
| 目标/特异性 | Detects mouse GPVI in direct ELISAs. In direct ELISAs, no cross-reactivity with recombinant human GPVI is observed. |
| 使用方法 | Flow Cytometry: 10 µL/106cells |
| 来源 | The product is shipped with polar packs. Upon receipt, store it immediately at the temperature recommended below. |
| 产品组分 |
| 供应商 | R&D Systems |
| Entrez Gene IDs | 51206 (Human); 243816 (Mouse) |
| 应用文献 | |
| R&D Systems personnel manually curate a database that contains references using R&D Systems products. The data collected includes not only links to publications in PubMed, but also provides information about sample types, species, and experimental conditions. Platelets from mice lacking the aryl hydrocarbon receptor exhibit defective collagen-dependent signaling. | |
| 纯化方式 | Protein A or G purified from hybridoma culture supernatant |
| 免疫原 | Mouse myeloma cell line NS0-derived recombinant mouse GPVI Gly24-Lys265 Accession # P0C191 |
| 生物活性 | Mouse |
| 标记 | Phycoerythrin |
| 背景 | GPVI (Platelet Glycoprotein VI), also known as Glycoprotein 5, is a member of the Ig superfamily. It is found on platelets and megakaryocytes, and serves as the main collagen receptor on platelets. Following exposure to subendothelial connective tissue, GPVI binds to a Gly-Pro-(hydroxy)Pro motif on collagen and generates a noncovalent membrane signaling complex with FcR gamma -chain. This interaction is stabilized by Integrin alpha 2 beta 1, followed by activation of PLC gamma 2 with clot initiation. Mature mouse GPVI is a 292 amino acid (aa) type I transmembrane protein. It possesses a 244 aa extracellular region (aa 22-265) that contains two C2-type Ig-like domains (aa 27-197)and two potential glycosylation sites, plus a 37 aa cytoplasmic tail (aa 287-313). There is one potential splice form that shows a deletion of aa 224-240. Over aa 24-265, mouse GPVI shares 70% and 86% aa identity with human and rat GPVI, respectively. |
| 运输条件 | Blue Ice |
| 存放说明 | 4℃ |
| 参考文献 |
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Detection of GPVI in Mouse Platelets by Flow Cytometry. Mouse platelets were stained with Rat Anti-Mouse GPVI PE‑conjugated Monoclonal Antibody (Catalog # FAB6758P, filled histogram) or isotype control antibody (Catalog # IC005P, open histogram). View our protocol for Staining Membrane-associated Proteins. |
