| 货号 | FAB3178P |
| 别名 | CD142 antigen; CD142; coagulation factor III (thromboplastin, tissue factor); Coagulation factor III; F3; FLJ17960; TF; TFA; Thromboplastin; Tissue Factor |
| 反应种属 | Mouse |
| 应用 | Flow Cytometry(10 µL/106cells) |
| 目标/特异性 | Detects mouse Coagulation Factor III in direct ELISAs and Western blots. In direct ELISAs and Western blots, approximately 5% cross‑reactivity with recombinant human Coagulation Factor III is observed. |
| 使用方法 | Flow Cytometry: 10 µL/106cells |
| 来源 | The product is shipped with polar packs. Upon receipt, store it immediately at the temperature recommended below. |
| 产品组分 |
| 供应商 | R&D Systems |
| Entrez Gene IDs | 2152 (Human); 14066 (Mouse) |
| 应用文献 | |
| R&D Systems personnel manually curate a database that contains references using R&D Systems products. The data collected includes not only links to publications in PubMed, but also provides information about sample types, species, and experimental conditions. Chimeric antigen receptor-modified T Cells inhibit the growth and metastases of established tissue factor-positive tumors in NOG mice | |
| 纯化方式 | Antigen Affinity-purified |
| 免疫原 | Mouse myeloma cell line NS0-derived recombinant mouse Coagulation Factor III/Tissue Factor Ala29-Glu251 Accession # P20352 |
| 生物活性 | Mouse |
| 标记 | Phycoerythrin |
| 背景 | Coagulation Factor III/Tissue Factor (TF), also known as thromboplastin and CD142, is an integral membrane protein found in a variety of cell types. It functions as a protein cofactor/receptor of Coagulation Factor VII, which is synthesized in the liver and circulated in the plasma (1). Upon binding of TF, the inactive factor VII is rapidly converted into activated VIIa. The resulting 1:1 complex of VIIa and TF initiates the coagulation pathway and has also important coagulation-independent functions such as angiognesis (2). Synthesized as a 294 amino acid precursor, mouse TF consists of a signal peptide (residues 1-28) and the mature chain (residues 29-294). As a type I membrane protein, it contains a transmembrane region (residues 252-274) and a cytoplasmic tail (residues 275-294) (3, 4). The purified rmTF corresponds to the ectodomain (residues 29-251) and is potent in activating thermolysin-processed rmCoagulation Factor VII (R&D Systems, Catalog # 3305-SE) under the conditions described in the Activity Assay Protocol. |
| 运输条件 | Blue Ice |
| 存放说明 | 4℃ |
| 参考文献 |
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Detection of Coagulation Factor III/ Tissue Factor in RAW 264.7 Mouse Cell Line by Flow Cytometry. RAW 264.7 mouse monocyte/macrophage cell line was stained with Goat Anti-Mouse Coagulation Factor III/Tissue Factor PE‑conjugated Antigen Affinity-purified Polyclonal Antibody (Catalog # FAB3178P, filled histogram) or isotype control antibody (Catalog # IC108P, open histogram). View our protocol for Staining Membrane-associated Proteins. |
