| 货号 | FAB3734G |
| 别名 | CD97 |
| 反应种属 | Mouse |
| 应用 | Flow Cytometry(5 µL/106cells) |
| 目标/特异性 | In direct ELISAs, approximately 20% cross-reactivity with recombinant mouse (rm) CD97 and no cross-reactivity with recombinant human (rh) CD96v2, rhCD97, or rmCD96 is observed. |
| 使用方法 | Flow Cytometry: 5 µL/106cells |
| 来源 | The product is shipped with polar packs. Upon receipt, store it immediately at the temperature recommended below. |
| 产品组分 |
| 供应商 | R&D Systems |
| Entrez Gene IDs | 976 (Human); 26364 (Mouse) |
| 纯化方式 | Protein A or G purified from hybridoma culture supernatant |
| 免疫原 | Chinese hamster ovary cell line CHO-derived recombinant mouse CD97v2 Gln24-His384 Accession # AAH06676 |
| 生物活性 | Mouse |
| 标记 | Alexa Fluor 488 |
| 背景 | CD97 is an approximately 90-100 kDa, 818 amino acid (aa) N-glycosylated member of the LNB-TM7 family of G protein-coupled proteins. Mouse CD97 contains a 510 aa N-terminal extracellular region with four EGF-like domains and a short 45 aa cytoplasmic tail. It undergoes proteolytic processing between Thr513-Ser514 to generate a 68-80 kDa N-terminal alpha -chain, and a 26-28 kDa membrane-bound beta -chain. The two chains remain non-covalently linked on the cell membrane with dissociation and solubilization of the alpha -chain under select conditions. CD97 in mouse has an alternately spliced variant 2 (V2) that exhibits a 94 aa deletion, resulting in the loss of the third EGF-like repeat (aa 69-119). CD97 is expressed on monocytes, macrophages, neutrophils, T cells, B cell subsets, dendritic cells, mesenchymal stem cells, and smooth muscle cells. It binds CD55/DAF, chondroitin sulfate, CD90, and alpha V beta 3 Integrin plus alpha 5 beta 1 Integrin. Interaction with these molecules may vary between splice forms of CD97. On the cell surface, the alpha beta heterodimer may "homodimerize", and form a true heterodimer with the 7-transmembrane LPAR1. CD97 participates in neutrophil migration, host defense, and angiogenesis. It also facilitates chemokine binding to PMNs, and inhibits select cell migration by downregulating MMP activity. Within aa 24-384, mouse CD97 variant 2 shares 51% aa and 76% aa sequence identity with corresponding regions of human and rat CD97, respectively. |
| 运输条件 | Blue Ice |
| 存放说明 | 4℃ |
| 参考文献 |
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Detection of CD97 in Mouse Splenocytes by Flow Cytometry. Mouse splenocytes were stained with Rat Anti-Mouse CD97 v2 Alexa Fluor® 488‑conjugated Monoclonal Antibody (Catalog # FAB3734G, filled histogram) or isotype control antibody (Catalog # IC005G, open histogram). View our protocol for Staining Membrane-associated Proteins. |
