Mouse L1CAM Phycoerythrin MAb (Clone 555) (100 TESTS)
货号：
FAB5674P 基本售价：
4094.7 元 规格：
100 Tests
产品信息
概述| 货号 | FAB5674P |
| 别名 | antigen identified by monoclonal R1; CAML1; CAML1N-CAML1; CD171 antigen; CD171; HSAS; HSAS1; L1 cell adhesion molecule; MASA; MIC5; N-CAM-L1; NCAM-L1; neural cell adhesion molecule L1; S10; SPG1 |
全称 | Cell Adhesion Molecule L1 | | 反应种属 | Mouse |
| 应用 | Flow Cytometry(10 µL/106cells)
|
| 目标/特异性 | Detects mouse L1CAM in direct ELISAs. In direct ELISAs, no cross-reactivity with recombinant human (rh) ALCAM, rhBCAM, rhEPCAM, rhMCAM, rhNCAM, rhNCAM-L1, rhOBCAM, recombinant mouse (rm)MAdCAM-1, or rmOCAM is observed. |
| 使用方法 | Flow Cytometry: 10 µL/106cells
|
| 来源 | The product is shipped with polar packs. Upon receipt, store it immediately at the temperature recommended below. |
| 产品组分 | |
性能| 供应商 | R&D Systems |
| Entrez Gene IDs | 3897 (Human); 16728 (Mouse); 50687 (Rat) |
| 纯化方式 | Protein A or G purified from hybridoma culture supernatant |
| 免疫原 | Mouse cerebellum-derived partially purified L1CAM |
| 生物活性 | Mouse |
| 标记 | Phycoerythrin |
| 背景 | L1CAM, also known as Neural Cell Adhesion Molecule L1 (NCAM-L1) and CD171, is a 200‑220 kDa type I transmembrane glycoprotein belonging to the immunoglobulin superfamily, L1/Neurofascin/NgCAM family of molecules. L1CAM is expressed by neurons, especially by growing axons on their growth cones. Non-neuronal cells such as Schwann cells, astrocytes, epithelial cells, and cells of myelomonocytic and lymphoid origin also express L1CAM. Mature mouse L1CAM consists of a 1104 amino acid (aa) extracellular domain (ECD) with 6 Ig-like domains and 5 fibronectin type-III domains, a 23 aa transmembrane region, and a 114 aa cytoplasmic tail. Mouse L1CAM shares 88% aa sequence identity with human L1CAM. L1CAM is critical for neural development. Specifically, L1CAM plays a key role in neuronal cell migration, axon outgrowth, axon fasciculation, synaptogenesis, and myelination. L1CAM mediates homophilic cell-cell interaction but also binds heterophilically with Axonin-1, CD24, CD9, Neurocan and several Integrins. L1CAM can undergo membrane‑proximal cleavage by ADAM10 and ADAM17, leading to the release of the soluble ECD and the generation of a membrane‑bound stub. The soluble ECD can serve as a substrate for integrin-mediated cell adhesion, thereby stimulating cellular motility and cell migration. L1CAM also plays a role in the ontogeny of human tumors, and its expression is linked to poor prognosis. Proteolytic processing by ADAM10 and presenilin/ gamma -secretase is essential for "forward signaling" where an L1CAM intracellular domain translocates to the nucleus and participates in gene regulation. Defects in L1CAM are the cause of the neurological MASA/CRASH syndrome. In addition, uncleaved L1CAM can cluster with Integrin alpha 5 either in-cis,orin-trans, inducing IL-1 beta expression and subsequent NF- kappa B activation. This is referred to as "reverse signaling". |
| 运输条件 | Blue Ice |
| 存放说明 | 4℃ |
| 参考文献 | - B Cell Chemotaxis, Migration, and Adhesion
- Cancer Biomarkers
- Cancer Stem Cell Markers
- Dendritic Cell Adhesion and Migration
- Epithelial Cell Markers and Intracellular Molecules
- Human CD Marker Products
- ICC / IHC Images: L1CAM
- Immunoglobulin Superfamily CAMs
- Monocyte Markers
- Neuronal Lineage Markers
- Semaphorins, Plexin Receptors, and Related Molecules
- Surface Ectodermal Cells
- Synaptic Adhesion Molecules
- Synaptic Proteins
- T Cell Migration/Adhesion
|
参考图片Detection of L1CAM in Mouse Splenocytes by Flow Cytometry. Mouse splenocytes were stained with Rat Anti-Mouse L1CAM PE‑conjugated Monoclonal Antibody (Catalog # FAB5674P, filled histogram) or isotype control antibody (Catalog # IC006P, open histogram). View our protocol for Staining Membrane-associated Proteins. |
