| 货号 | IC5294R-100 |
| 别名 | APG5 autophagy 5-like (S. cerevisiae); APG5; APG5LS. cerevisiae)-like; ASP; ASPAPG5-LIKE; ATG5 autophagy related 5 homolog (S. cerevisiae); ATG5; Autophagy protein 5 | 全称 | ATG5 Autophagy Related 5 Homolog |
| 应用 | Intracellular Staining by Flow Cytometry |
| 目标/特异性 | Detects human, mouse, and rat ATG5 in Western blots. |
| 使用方法 | Intracellular Staining by Flow Cytometry: 5 µL/106cells |
| 来源 | The product is shipped with polar packs. Upon receipt, store it immediately at the temperature recommended below. |
| 产品组分 |
| 供应商 | R&D Systems |
| Entrez Gene IDs | 9474 (Human); 11793 (Mouse) |
| 纯化方式 | Protein A or G purified from hybridoma culture supernatant |
| 免疫原 | E. coli-derived recombinant human ATG5 Asn99-Thr193 Accession # Q9H1Y0 |
| 生物活性 | Human, Mouse, Rat |
| 标记 | Alexa Fluor 647 |
| 背景 | ATG5 (Autophagy-related Protein), also known as APG5L and Apoptosis-specific Protein, is a ubiquitous 32 kDa member of the ATG family of proteins. ATG5 exists as a covalent heterodimer with ATG12 through the creation of a Lys-Gly linkage. The ATG5:ATG12 heterodimer associates noncovalently with an ATG16 multimer to generate autophagosomes. Human ATG5 is 275 amino acids in length and contains N- and C-terminal ubiquitin-like domains (aa 15‑105 and 187‑275) separated by a helix-rich linker region that contains a dimerizing Lys at position 130. There are two potential alternate start sites at Met80 and Met173. Over aa 99‑193, human ATG5 is 97% aa identical to mouse ATG5. |
| 运输条件 | Blue Ice |
| 存放说明 | 4℃ |
| 参考文献 |
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Detection of ATG5 in HeLa Human Cell Line by Flow Cytometry. HeLa human cervical epithelial carcinoma cell line was stained with Mouse Anti-Human/Mouse/Rat ATG5 Alexa Fluor® 647‑conjugated Monoclonal Antibody (Catalog # IC5294R, filled histogram) or isotype control antibody (Catalog # IC0041R, open histogram). To facilitate intracellular staining, cells were fixed and permeabilized with FlowX FoxP3 Fixation & Permeabilization Buffer Kit (Catalog # FC012). View our protocol for Staining Intracellular Molecules. |
