| 货号 | IC6608R-100 |
| 别名 | APG7 autophagy 7-like (S. cerevisiae); APG7; APG7L; APG7-LIKE; ATG12-activating enzyme E1 ATG7; ATG7 autophagy related 7 homolog (S. cerevisiae); ATG7; Autophagy-related protein 7; DKFZp434N0735; GSA7; hAGP7; ubiquitin activating enzyme E1-like protein; Ubiquitin-activating enzyme E1-like protein; ubiquitin-like modifier-activating enzyme ATG7 | 全称 | ATG7 Autophagy Related 7 Homolog |
| 应用 | Intracellular Staining by Flow Cytometry |
| 目标/特异性 | Detects human ATG7 in direct ELISAs and Western blots.In direct ELISAs and Western blots, nocross-reactivity with recombinant human ATG3, 4A, 4B, 5, 10, 12, or 16L1 isobserved. |
| 使用方法 | Intracellular Staining by Flow Cytometry: 5 µL/106cells |
| 来源 | The product is shipped with polar packs. Upon receipt, store it immediately at the temperature recommended below. |
| 产品组分 |
| 供应商 | R&D Systems |
| Entrez Gene IDs | 74244 (Mouse); 312647 (Rat); 10533 (Yeast); 10533 (Yeast) |
| 纯化方式 | Protein A or G purified from hybridoma culture supernatant |
| 免疫原 | E. coli-derived recombinant human ATG7 Asn387-Gln570 Accession # O95352 |
| 生物活性 | Human, Mouse |
| 标记 | Alexa Fluor 647 |
| 背景 | ATG7, also known as APG7, is a 78 kDa cytosolic ubiquitin-E1-like enzyme that plays a key role in the autophagic pathway of intracellular bulk degradation. It is required for the conjugation of ATG5 to ATG12, the lipidation of ATG8, and subsequent autophagosome formation. ATG7 is required for mitochondrial removal during erythropoiesis and for the maintenance of axonal homeostasis. Alternate splicing of human ATG7 generates an isoform that lacks 31 aa at the C-terminus, a region that is required for ATG8 lipidation. Human ATG7 shares 93% aa sequence identitiy with mouse and rat ATG7. |
| 运输条件 | Blue Ice |
| 存放说明 | 4℃ |
| 参考文献 |
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Detection of ATG7 in HeLa Human Cell Line by Flow Cytometry. HeLa human cervical epithelial carcinoma cell line was stained with Mouse Anti-Human/Mouse ATG7 Alexa Fluor® 647‑conjugated Monoclonal Antibody (Catalog # IC6608R, filled histogram) or isotype control antibody (Catalog # IC002R, open histogram). To facilitate intracellular staining, cells were fixed and permeabilized with FlowX FoxP3 Fixation & Permeabilization Buffer Kit (Catalog # FC012). View our protocol for Staining Intracellular Molecules. |
