货号 | FAB65181A |
别名 | CD34 antigenhematopoietic progenitor cell antigen CD34; CD34 molecule; CD34; HPCA1 |
反应种属 | Mouse |
应用 | Flow Cytometry(10 µL/106cells) |
目标/特异性 | Detects mouse CD34 in direct ELISAs and Western blots. In direct ELISAs, less than 3% cross-reactivity with recombinant porcine CD34 is observed. |
使用方法 | Flow Cytometry: 10 µL/106cells |
来源 | The product is shipped with polar packs. Upon receipt, store it immediately at the temperature recommended below. |
产品组分 |
供应商 | R&D Systems |
Entrez Gene IDs | 947 (Human); 12490 (Mouse); 305081 (Rat); 397160 (Porcine); 415130 (Canine) |
纯化方式 | Antigen Affinity-purified |
免疫原 | Mouse myeloma cell line NS0-derived recombinant mouse CD34 Thr35-Thr287 Accession # Q64314 |
生物活性 | Mouse |
标记 | Allophycocyanin |
背景 | Mouse and rat CD34 is a 80 -100 kDa member of the CD34/podocalyxin family of molecules. It is a sialomucin type glycoprotein, and presents carbohydrate to selectins during cell migration. CD34 is found on mast cells, eosinophils, vascular endothelial cells, CD34+ stem cells and renal mesangial cells. Mature mouse CD34 is a 348 amino acid (aa) type I transmembrane protein (aa 35-382). It contains a 253 aa extracellular region (aa 35-287), and a 74 aa cytoplasmic domain that undergoes Tyr phosphorylation. Mouse and rat CD34 also is likely to undergo membrane cleavage that will generate a 54 kDa soluble form. Over aa 35-287, mouse CD34 shares 57% and 82% aa identity with human and rat Cd34, respectively. |
运输条件 | Blue Ice |
存放说明 | 4℃ |
参考文献 |
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Detection of CD34 in bEnd.3 Mouse Cell Line by Flow Cytometry. bEnd.3 mouse endothelioma cell line was stained with Sheep Anti-Mouse CD34 APC‑conjugated Antigen Affinity-purified Polyclonal Antibody (Catalog # FAB65181A, filled histogram) or isotype control antibody (Catalog # IC016A, open histogram). View our protocol for Staining Membrane-associated Proteins. |