货号 | FAB27881G |
别名 | CMRF35-like molecule 1; CD300 molecule-like family member f; CD300f; CD300LF; CLIM1; CLM1; CLM-1; DIgR2; IGSF13; IREM1; IREM-1; LMIR3; NKIR; Pigr3 | 全称 | Leukocyte Mono Ig-like Receptor 3 |
反应种属 | Mouse |
应用 | Flow Cytometry(5 µL/106cells) |
目标/特异性 | Detects mouse CD300f/LMIR3 in direct ELISAs and Western blots. In direct ELISAs, no cross-reactivity with recombinant mouse (rm) LMIR4, rmCD300b/LMIR5, recombinant human (rh) CD300a/LMIR1, rhCD300c/LMIR2, rhCD300f/LMIR3, or rhCD300e/LMIR6 is observed. |
使用方法 | Flow Cytometry: 5 µL/106cells |
来源 | The product is shipped with polar packs. Upon receipt, store it immediately at the temperature recommended below. |
产品组分 |
供应商 | R&D Systems |
Entrez Gene IDs | 146722 (Human); 246746 (Mouse) |
纯化方式 | Protein A or G purified from hybridoma culture supernatant |
免疫原 | Mouse myeloma cell line NS0-derived recombinant mouse CD300f/LMIR3 Cys16-Gly188 Accession # Q6SJQ7 |
生物活性 | Mouse |
标记 | Alexa Fluor 488 |
背景 | Mouse LMIR3, also known as CLM-1, IgSF13, IREM-1 and CMRF35-like Molecule 1, and designated CD300f, is a 55 kDa type I transmembrane (TM) glycoprotein. It is synthesized as a 283 amino acid (aa) precursor that contains a putative 19 aa signal sequence, a 175 aa extracellular region that contains one Ig-like domain, a 17 aa TM segment and a 72 aa cytoplasmic domain that contains an ITIM sequence. There are multiple alleles for this gene that vary considerably in size. Mouse LMIR3 is expressed in monocytic cell lines. Mature mouse LMIR3 extracellular region shares 43% and 72% aa identity with human and rat LMIR3 extracellular region, respectively. |
运输条件 | Blue Ice |
存放说明 | 4℃ |
参考文献 |
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Detection of CD300f/LMIR3 in RAW 264.7 Mouse Cell Line by Flow Cytometry. RAW 264.7 mouse monocyte/macrophage cell line was stained with Rat Anti-Mouse CD300f/LMIR3 Alexa Fluor® 488‑conjugated Monoclonal Antibody (Catalog # FAB27881G, filled histogram) or isotype control antibody (Catalog # IC005G, open histogram). View our protocol for Staining Membrane-associated Proteins. |