Mouse CD28 Alexa Fluor 700 Antibody (Clone 794716) (100 UG)
货号：
FAB4832N-100UG 基本售价：
3817.0 元 规格：
100 UG
产品信息
概述| 货号 | FAB4832N-100UG |
| 别名 | CD28 antigen (Tp44); CD28 antigen; CD28 molecule; CD28; MGC138290; T-cell-specific surface glycoprotein CD28; Tp44 |
| 应用 | Flow Cytometry |
| 目标/特异性 | Detects mouse CD28 in ELISA. |
| 使用方法 | Flow Cytometry: 0.25-1 µg/106cells
|
| 来源 | The product is shipped with polar packs. Upon receipt, store it immediately at the temperature recommended below. |
| 产品组分 | |
性能| 供应商 | R&D Systems |
| Entrez Gene IDs | 940 (Human); 12487 (Mouse); 102146670 (Cynomolgus Monkey) |
| 免疫原 | Mouse myeloma cell line NS0-derived recombinant mouse CD28
Accession # P31041 |
| 生物活性 | Mouse |
| 标记 | Alexa Fluor 700 |
| 背景 | CD28 and CTLA-4, together with their ligands B7-1 and B7-2, constitute one of the dominant costimulatory pathways that regulate T and B cell responses. CD28 and CTLA-4 are structurally homologous molecules that are members of the immunoglobulin (Ig) gene superfamily. Both CD28 and CTLA-4 are composed of a single Ig
V‑like extracellular domain, a transmembrane domain and an intracellular domain. CD28 and CTLA-4 are both expressed on the cell surface as disulfide-linked homodimers or as monomers. The genes encoding these two molecules are closely linked on human chromosome 2 and mouse chromosome 1. Mouse CD28 is expressed constitutively on virtually 100% of mouse T cells and on developing thymocytes. Cell surface expression of mouse CD28 is down-regulated upon ligation of CD28 in the presence of PMA or PHA. In contrast, CTLA-4 is not expressed constitutively but is upregulated rapidly following T cell activation and CD28 ligation. Cell surface expression of CTLA-4 peaks approximately 48 hours after activation. Although both CTLA-4 and CD28 can bind to the same ligands, CTLA-4 binds to
B7‑1 and B7‑2 with a 20-100 fold higher affinity than CD28. CD28/B7 interaction has been shown to prevent apoptosis of activated T cells via the up-regulation of
Bcl‑xL. CD28 ligation has also been shown to regulate Th1/Th2 differentiation. Agonist activity has been reported using MAB4831 (4,5). |
| 运输条件 | Blue Ice |
| 存放说明 | 4℃ |
| 参考文献 | - Lenschow, D.J.et al. (1996) Annu. Rev. Immunol. 14:233.
- Hathcock, K.S. and R.J. Hodes (1996) Advances in Immunol. 62:131.
- Ward, S.G. (1996) Biochem. J. 318:361.
- Nguyen, P. et al. (2003) Blood 13:4320.
- Orbach, A. et al. (2007) J. Immunol. 179:7287.
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