| 货号 | FAB19781U-100UG |
| 别名 | EC 5.3.2.1; EC 5.3.3.12; GIFmacrophage migration inhibitory factor; GLIF; Glycosylation-inhibiting factor; L-dopachrome isomerase; L-dopachrome tautomerase; macrophage migration inhibitory factor (glycosylation-inhibiting factor); MIF; MMIF; Phenylpyruvate tautomerase | 全称 | Macrophage Migration Inhibitory Factor |
| 应用 | Flow Cytometry |
| 目标/特异性 | Detects mouse MIF in ELISA and Western Blot. |
| 使用方法 | Flow Cytometry: 0.25-1 µg/106cells |
| 来源 | The product is shipped with polar packs. Upon receipt, store it immediately at the temperature recommended below. |
| 产品组分 |
| 供应商 | R&D Systems |
| Entrez Gene IDs | 4282 (Human); 17319 (Mouse) |
| 免疫原 | E. coli-derived recombinant mouse MIF Met1-Ala115 Accession # P34884 |
| 生物活性 | Mouse, Rat |
| 标记 | Alexa Fluor 350 |
| 背景 | MIF (Macrophage Inhibitory Factor; also GIF) is a 12-13 kDa member of the MIF family of proteins. Although secreted, it possesses no definitive signal sequence. MIF is widely expressed and serves to regulate immune reactions. On macrophages and B cells, MIF binds to a CD74:CD44 complex, initiating downstream signaling. On monocytes, this promotes TNF alpha production plus IFN-gamma -stimulated NO production. On B cells, this promotes B cell survival. MIF also binds to leukocyte CD74:CXCR2 and CD74:CXCR4 heterodimers, initiating T cell and monocyte migration. A tautomerase activity has also been shown for MIF and is suggested to involve the entire length of the molecule. Mouse MIF is 114 amino acids (aa) in length. MIF is phosphorylated at Ser91, and an additional, singular Cys residue is covalently linked to MIF at Cys60. MIF is considered to act as a homotrimer. Full-length mouse MIF shows 90% and 99% aa sequence identity with human and rat MIF, respectively. |
| 运输条件 | Blue Ice |
| 存放说明 | 4℃ |
| 参考文献 |
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