| 货号 | IC1249R-100UG |
| 别名 | GCG; glicentin-related polypeptide; Glucagon; GRPP |
| 应用 | Intracellular Staining by Flow Cytometry |
| 目标/特异性 | Detects human and mouse Glucagon. |
| 使用方法 | Intracellular Staining by Flow Cytometry: 0.25-1 µg/106cells |
| 来源 | The product is shipped with polar packs. Upon receipt, store it immediately at the temperature recommended below. |
| 产品组分 |
| 供应商 | R&D Systems |
| Entrez Gene IDs | 2641 (Human); 14526 (Mouse); 24952 (Rat) |
| 纯化方式 | Protein A or G purified from hybridoma culture supernatant |
| 免疫原 | Human Glucagon synthetic peptide |
| 生物活性 | Human, Mouse |
| 标记 | Alexa Fluor 647 |
| 背景 | Glucagon is a 29 amino acid residue pancreatic peptide hormone. Itstimulates gluconeogenesis and glycogenolysis to counter theglucose-lowering action of insulin. |
| 运输条件 | Blue Ice |
| 存放说明 | 4℃ |
| 参考文献 |
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Detection of Glucagon in beta TC-6 Mouse Cell Line by Flow Cytometry. beta TC-6 mouse beta cell insulinoma cell line was stained with Alexa Fluor® 647-conjugated Mouse Anti-Human/Mouse Glucagon Monoclonal Antibody (Catalog # IC1249R, filled histogram) or isotype control antibody (Catalog # IC003R, open histogram). To facilitate intracellular staining, cells were fixed with Flow Cytometry Fixation Buffer (Catalog # FC004) and permeabilized with Flow Cytometry Permeabilization/Wash Buffer I (Catalog # FC005). View our protocol for Staining Intracellular Molecules. |
