| 货号 | FAB1755S-100UG |
| 别名 | BAFF R; BAFFR; BR3; CD268; TNFRSF13C | 全称 | B cell Activating Factor Receptor |
| 应用 | Flow Cytometry |
| 目标/特异性 | Detects mouse BAFF R/TNFRSF13C in direct ELISAs. In direct ELISAs, 100% cross-reactivity with recombinant human (rh) BAFF R is observed, approximately 10‑25% cross-reactivity with recombinant mouse (rm) 4‑1BB, rmDR3, rhDR6, rmEDAR, rhHVEM, rmNGF R, rmOX40, and rmTROY is observed, and no cross-reactivity with rmCD27, rmCD30, rmCD40, rmFAS, rmGITR, rmLymphotoxin beta R, rmOPG, rmRANK, rmTNFRI, or rmTNF RII is observed. |
| 使用方法 | Flow Cytometry: 0.25-1 µg/106cells |
| 来源 | The product is shipped with polar packs. Upon receipt, store it immediately at the temperature recommended below. |
| 产品组分 |
| 供应商 | R&D Systems |
| Entrez Gene IDs | 115650 (Human); 72049 (Mouse) |
| 免疫原 | Mouse myeloma cell line NS0-derived recombinant mouse BAFF R/TNFRSF13C Gly2-Ala71 Accession # Q9D8D0 |
| 生物活性 | Mouse |
| 标记 | Alexa Fluor 750 |
| 背景 | B-cell activating factor (BAFF), also known as BlyS, TALL-1, TNAK, and zTNF4, is a TNF ligand superfamily member and has been designated TNFSF13B. It is produced by macrophages, dendritic cells, and T lymphocytes. BAFF promotes the survival of B cells and is essential for B cell maturation (1‑4). BAFF binds to three TNF receptor superfamily members: B‑cell maturation antigen (BCMA/TNFRSF17), transmembrane activator and calcium-modulator and cyclophilin ligand interactor (TACI/TNFRSF13B) and BAFF receptor (BAFF R/BR3/TNFRSF 13C). These receptors are type III transmembrane proteins that lack a signal peptide. Whereas TACI and BCMA bind BAFF and another TNF superfamily ligand, APRIL(a proliferation-inducing ligand), BAFF-R selectively binds BAFF. Mouse BAFF-R cDNA encodes a 175 amino acid residue (aa) transmembrane protein with a 71 aa extracellular domain, a 21 aa transmembrane domain, and a 83 aa cytoplasmic region. A second isoform of BAFF R that has a 72 aa cytoplamic region can also be produced by alternative splicing. The BAFF R extracellular domain lacks the TNF receptor canonical cysteine-rich domain (CRD) and contains only a partial CRD with four cysteine residues. Human and mouse BAFF R share 56% aa sequence identity. BAFF R is highly expressed in spleen, lymph node and resting B cells. It is also expressed at lower levels in activated B cells, in resting CD4+T cells, in thymus and peripheral blood leukocytes. BAFF knockout mice lack mature B cells and has profound defects in antibody mediated immune responses. Similarly, A/WySnJ mice that are defective in BAFF R intracellular signaling also lack mature B cells, suggesting that BAFF R is the critical receptor for BAFF during B lymphopoiesis. In contrast, BCMA- or TACI-deficient mice have no major defect in B‑cell development. While the function of BCMA is not defined, TACI has been shown to control |
| 运输条件 | Blue Ice |
| 存放说明 | 4℃ |
| 参考文献 |
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