Human/Mouse FoxP3 Phycoerythrin MAb (Clone 1054C) (100 TESTS)【科瑞奥博】

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Human/Mouse FoxP3 Phycoerythrin MAb (Clone 1054C) (100 TESTS)

货号： IC8214P-100 基本售价： 3817.0 元规格： 100 Tests

产品信息

概述
货号	IC8214P-100
简介	Recombinant Monoclonal Antibody. Use with Catalog # FC012, FlowX FoxP3 Fixation & Permeabilization Buffer Kit
别名	AIID; AIIDMGC141961; DIETER; forkhead box P3; Forkhead Box Protein P3; FOXP3delta7; immune dysregulation, polyendocrinopathy, enteropathy, X-linked; Immunodeficiency, Polyendocrinopathy, Enteropathy, X-Linked; IPEX; JM2; MGC141961; MGC141963; PIDX; PIDXMGC141963; SCURFIN; XPID; XPIDpolyendocrinopathy, enteropathy, X-linked
全称	Forkhead Box P3
反应种属	Human/Mouse
应用	Intracellular Staining by Flow Cytometry
目标/特异性	Detects human and mouse FoxP3 in flow cytometry.
使用方法	Intracellular Staining by Flow Cytometry: 10 µL/10⁶cells
来源	The product is shipped with polar packs. Upon receipt, store it immediately at the temperature recommended below.
产品组分

性能
供应商	R&D Systems
Entrez Gene IDs	50943 (Human); 20371 (Mouse)
纯化方式	Protein A or G purified from cell culture supernatant
免疫原	E. coli-derived recombinant human FoxP3 Met1-Leu71 Accession # Q9BZS1
生物活性	Human, Mouse
标记	Phycoerythrin
背景	Human FoxP3 is a 47 kDa member of the P subclass of the FOX (forkhead box) family of transcription factors. It contains a Leu-rich repeat, a C2H2 zinc finger region, and a C-terminal FKH (fork head), DNA-binding domain. Three isoforms for FoxP3 have been reported. All three isoforms share the sequence used as the immunogen. FoxP3 directly associates with NFAT and NFkB, suppressing their activity in CD4⁺ T cells. In human, FoxP3 is found in CD4⁺, CD8⁺ and CD4⁺CD25⁺ T cells. Over the region used for immunization of the amino acid sequence, mouse FoxP3 is 83% to 88% identical to rat, human, canine, and bovine FoxP3.
运输条件	Blue Ice
存放说明	4℃
参考文献	Forkhead Domain Transcription Factors ICC / IHC Images: FoxP3 Multiple Sclerosis and Myelin Related Molecules Regulatory T Cells (Tregs) Transcription Factors and Regulators Transcription Factors in the Akt Pathway Transcriptional Regulators in Allergy and Asthma

参考图片

Detection of FoxP3 in Human PBMC Natural Tregs by Flow Cytometry. Human peripheral blood mononuclear cell (PBMC) natural regulatory T cells (Tregs) were surface stained with (A) Mouse Anti-Human CD4 Fluorescein-conjugated Monoclonal Antibody (Catalog # FAB3791F) and (B) Mouse Anti-Human IL‑2 R alpha (CD25) APC-conjugated Monoclonal Antibody (Catalog # FAB1020A), followed by intracellular staining using Rabbit Anti-Human/Mouse FoxP3 PE‑conjugated Monoclonal Antibody (Catalog # IC8214P). To facilitate intracellular staining, cells were fixed and permeabilized with FlowX FoxP3 Fixation & Permeabilization Buffer Kit (Catalog # FC012). Cells were gated on lymphocytes.

Detection of FoxP3 in C57BL/6 Mouse Splenocyte Natural Tregs by Flow Cytometry. C57BL/6 mouse splenocyte natural regulatory T cells (Tregs) were surface stained with (A) Rat Anti-Mouse CD4 APC‑conjugated Monoclonal Antibody (Catalog # FAB554A) and (B) Rat Anti-Mouse IL‑2 R alpha (CD25) APC‑conjugated Monoclonal Antibody (Catalog # FAB2438A) followed by intracellular staining using Rabbit Anti-Human/Mouse FoxP3 PE-conjugated Monoclonal Antibody (Catalog # IC8214P). To facilitate intracellular staining, cells were fixed and permeabilized with FlowX FoxP3 Fixation & Permeabilization Buffer Kit (Catalog # FC012). Cells were gated on lymphocytes.