| 货号 | FAB3905P |
| 别名 | 12E7; antigen identified by monoclonal antibodies 12E7, F21 and O13; CD99 antigenY homolog; CD99 molecule; CD99; E2 antigen; HBA71; MIC2 (monoclonal 12E7); MIC2; MIC2Y; MSK5X; pilr-1; PILR-L; Protein MIC2; surface antigen MIC2; T-cell surface glycoprotein E2 |
| 反应种属 | Mouse |
| 应用 | Flow Cytometry(10 µL/106cells) |
| 目标/特异性 | Detects mouse CD99 in direct ELISAs and Western blots. In direct ELISAs, less than 1% cross-reactivity with recombinant mouse (rm) PILR‑ alpha and rmPILR‑ beta is observed. |
| 使用方法 | Flow Cytometry: 10 µL/106cells |
| 来源 | The product is shipped with polar packs. Upon receipt, store it immediately at the temperature recommended below. |
| 产品组分 |
| 供应商 | R&D Systems |
| Entrez Gene IDs | 4267 (Human); 673094 (Mouse); 652929 (Rat) |
| 纯化方式 | Antigen Affinity-purified |
| 免疫原 | Mouse myeloma cell line NS0-derived recombinant mouse CD99 Gly25-Leu138 Accession # Q8VCN6 |
| 生物活性 | Mouse |
| 标记 | Phycoerythrin |
| 背景 | CD99, also known as PILR-L and MIC2, is a 125 amino acid (aa), 24‑30 kDa glycosylated transmembrane glycoprotein expressed as a homodimer on the surface of endothelial, T and B cells, monocytes and stem cells. CD99 has been implicated in processes like homotypic cell adhesion, diapedesis and oncosuppression. The extracellular domain of the mouse CD99 protein used as immunogen has 60% aa sequence identity to rat CD99 and 43% aa sequence identity to the human CD99. |
| 运输条件 | Blue Ice |
| 存放说明 | 4℃ |
| 参考文献 |
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Detection of CD99 in Mouse Splenocytes by Flow Cytometry. Mouse splenocytes were stained with Goat Anti-Mouse CD99 PE‑conjugated Antigen Affinity-purified Polyclonal Antibody (Catalog # FAB3905P, filled histogram) or isotype control antibody (Catalog # IC108P, open histogram). View our protocol for Staining Membrane-associated Proteins. |
