| 货号 | FAB5994P |
| 别名 | BDCA4; BDCA-4; CD304 antigen; CD304; DKFZp686A03134; DKFZp781F1414; neuropilin 1; neuropilin-1; NRP1; NRPNP1; transmembrane receptor; Vascular endothelial cell growth factor 165 receptor; VEGF165RCD304 |
| 反应种属 | Mouse |
| 应用 | Flow Cytometry(10 µL/106cells) |
| 目标/特异性 | Detects mouse Neuropilin-1 in direct ELISAs. In direct ELISAs, less than 5%cross-reactivity with recombinant rat (rr) Neuropilin-1 is observed and nocross-reactivity with recombinant human (rh) Neuropilin-1, rhNeuropilin-2, orrrNeuropilin-2 is observed. |
| 使用方法 | Flow Cytometry: 10 µL/106cells |
| 来源 | The product is shipped with polar packs. Upon receipt, store it immediately at the temperature recommended below. |
| 产品组分 |
| 供应商 | R&D Systems |
| Entrez Gene IDs | 8829 (Human); 18186 (Mouse); 246331 (Rat) |
| 应用文献 | |
| R&D Systems personnel manually curate a database that contains references using R&D Systems products. The data collected includes not only links to publications in PubMed, but also provides information about sample types, species, and experimental conditions. High Fat Diet Inhibits Dendritic Cell and T Cell Response to Allergens but Does Not Impair Inhalational Respiratory Tolerance | |
| 纯化方式 | Protein A or G purified from hybridoma culture supernatant |
| 免疫原 | Mouse myeloma cell line NS0-derived recombinant mouse Neuropilin-1 Phe21-Pro856 Accession # P97333 |
| 生物活性 | Mouse |
| 标记 | Phycoerythrin |
| 背景 | Neuropilin-1 (Nrp-1, previously neuropilin; also CD304) is a 130 - 140 kDa type I transmembrane (TM) glycoprotein that regulates axon guidance and angiogenesis. The full-length 923 amino acid (aa) mouse Nrp-1 contains a 623 aa extracellular domain (ECD) that shares 98% aa identity with rat and 93% aa identity with human, equine, bovine and canine Nrp-1. The ECD contains two N-terminal CUB domains (termed a1a2), two domains with homology to coagulation factors V and VIII (b1b2) and a MAM (meprin) domain (c). At least one splice variant that diverges at aa 587 and lacks the TM domain has been sequenced. This form is potentially a soluble antagonist, based on results from human Nrp-1 splice variants. The sema domains of Class III secreted semaphorins such as Sema3A bind Nrp-1 a1a2. Heparin, the heparin-binding forms of VEGF (VEGF165, VEGF-B and VEGF-E), PlGF (PlGF2), plus the C-terminus of Sema3 bind the b1b2 region of Nrp-1. Nrp-1 and Nrp-2 share 48% aa identity within the ECD and can form homo- and hetero-oligomers via interaction of their MAM domains. Neuropilins show partially overlapping expression in neuronal and endothelial cells during development. Both neuropilins act as co-receptors with plexins, including plexins A1, A4, B1, and D1, to bind class III semaphorins, such as Sema 3A, 3C, 3D, 3E, and 3F, that often mediate axon repulsion. Both Nrp-1 and Nrp-2 serve as co-receptors with VEGF R2 (also called KDR or Flk-1) for VEGF165 binding. Sema3A signaling can be blocked by VEGF165, which has higher affinity for Npn-1. Nrp-1 is preferentially expressed in developing or remodeling arteries. Nrp-1 is also expressed on dendritic cells and mediates DC-induced T cell proliferation. |
| 运输条件 | Blue Ice |
| 存放说明 | 4℃ |
| 参考文献 |
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Detection of Neuropilin‑1 in bEnd.3 Mouse Cell Line by Flow Cytometry. bEnd.3 mouse endothelioma cell line was stained with Rat Anti-Mouse Neuropilin‑1 PE‑conjugated Monoclonal Antibody (Catalog # FAB5994P, filled histogram) or isotype control antibody (Catalog # IC006P, open histogram). View our protocol for Staining Membrane-associated Proteins. |
