Mouse SIGNR1/CD209b Phycoerythrin Affinity Purified PAb (100 TESTS)【科瑞奥博】

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Mouse SIGNR1/CD209b Phycoerythrin Affinity Purified PAb (100 TESTS)

货号： FAB1836P 基本售价： 3529.5 元规格： 100 Tests

产品信息

概述
货号	FAB1836P
别名	CD209 antigen like protein B; CD209b; DC SIGN related protein 1; DC SIGNR1; OtB7
全称	SIGN-related 1
反应种属	Mouse
应用	Flow Cytometry(10 µL/10⁶cells)
目标/特异性	Detects mouse SIGNR1 in direct ELISAs and Western blots. In Western blots, this antibody shows approximately 5% cross‑reactivity with recombinant mouse SIGNR4.
使用方法	Flow Cytometry: 10 µL/10⁶cells
来源	The product is shipped with polar packs. Upon receipt, store it immediately at the temperature recommended below.
产品组分

性能
供应商	R&D Systems
Entrez Gene IDs	69165 (Mouse)
纯化方式	Antigen Affinity-purified
免疫原	Mouse myeloma cell line NS0-derived recombinant mouse SIGNR1 Ser76-Gly325 Accession # Q8CJ91
生物活性	Mouse
标记	Phycoerythrin
背景	SIGNR1 belongs to the family of C-type lectins that participate in innate immune responses by binding and clearing pathogens (1-3). SIGNR1 is one of four mouse proteins, encoded by separate genes, with significant homology to DC-SIGN and DC-SIGNR (4). Mouse SIGNR1 consists of a 53 amino acid (aa) cytoplasmic domain, a 20 aa transmembrane segment, and a 252 aa extracellular domain that contains a juxtamembrane neck region and a carbohydrate recognition domain (CRD) (4, 5). SIGNR1 is a 50kDa type II protein that forms high molecular weight aggregates as has been shown for DC-SIGN and DC-SIGNR (6, 7). The mouse SIGNR1 mRNA is extensively spliced, giving rise to multiple isoforms in addition to variability in the 5’ and 3’ untranslated regions. The alpha isoform is known as SIGNR1. The beta and gamma isoforms lack the transmembrane segment, with the gamma isoform also showing a 21 aa insertion in the CRD. The δ isoform lacks almost the entire CRD (5). Functional differences between the isoforms have not been described. Within the CRD, mouse SIGNR1 shares 67%-70% aa sequence identity with mouse DC‑SIGN, SIGNR2, 3, and 4, and 64%-68% aa sequence identity with human DC-SIGN, human L-SIGN, and rat DC-SIGN. The CRD binds mannose, GlcNAc, and fucose structures on some bacterial strains, mycobacteria, and yeast (8). SIGNR1 is expressed on macrophages found in the splenic marginal zone, lymph node medulla, and peritoneum (6, 9, 10). It plays a dominant role in the clearance of circulating S. pneumoniae (11-13), enhances TLR4 signaling (14), and cooperates with Dectin-1 in the nonopsonic clearance of fungal pathogens (9, 15). SIGNR1 also binds mouse ICAM-2, human ICAM-2 and -3, and HIV gp120 (10).
运输条件	Blue Ice
存放说明	4℃
参考文献	Marshall, A.S.J. and S. Gordon (2004) Eur. J. Immunol. 34:18. Cambi, A. and C.G. Figdor (2005) Curr. Opin. Immunol. 17:345. McGreal, E.P. et al. (2005) Curr. Opin. Immunol. 17:18. Park, C.G. et al. (2001) Int. Immunol. 13:1283. Parent, S.A. et al. (2002) Gene 293:33. Kang, Y.-S. et al. (2003) Int. Immunol. 15:177. Feinberg, H. et al. (2005) J. Biol. Chem. 280:1327. Galustian, C. et al. (2003) Int. Immunol. 16:853. Taylor, P.R. et al. (2004) J. Immunol. 172:1157. Geijtenbeek, T.B.H. et al. (2002) Blood 100:2908. Koppel, E.A. et al. (2005) Eur. J. Immunol. 35:2962. Lanoue, A. et al. (2004) J. Exp. Med. 200:1383. Kang, Y.-S. et al. (2004) Proc. Natl. Acad. Sci. 101:215. Nagaoka, K. et al. (2005) Int. Immunol. 17:827. Takahara, K. et al. (2003) Int. Immunol. 16:819.

参考图片

Detection of SIGNR1/CD209b in Mouse Splenocytes by Flow Cytometry. Mouse splenocytes were stained with Rat Anti-Mouse B220/CD45R APC‑conjugated Monoclonal Antibody (Catalog # FAB1217A) and either (A) Goat Anti-Mouse SIGNR1/CD209b PE‑conjugated Antigen Affinity-purified Polyclonal Antibody (Catalog # FAB1836P) or (B) Normal Goat IgG Phycoerythrin Control (Catalog # IC108P). View our protocol for Staining Membrane-associated Proteins.