Human/Mouse IL-12/IL-35 p35 Fluorescein MAb (Clone 27537) (100 TESTS)【科瑞奥博】

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Human/Mouse IL-12/IL-35 p35 Fluorescein MAb (Clone 27537) (100 TESTS)

货号： IC2191F 基本售价： 3529.5 元规格： 100 Tests

产品信息

概述
货号	IC2191F
别名	IL-12A
全称	Interleukin 12 p35
反应种属	Human/Mouse
应用	Intracellular Staining by Flow Cytometry(10 µL/10⁶cells)
目标/特异性	Detects human IL‑12/IL-35 p35 in direct ELISAs and Western blots. Detects the p35 subunit either as part of a p40/p35 heterodimer or as a free subunit after reduction of the heterodimer. This antibody does not recognize IL-12 p40 homodimers but shows strong cross-reactivity with the p35 subunits from porcine and mouse systems.
使用方法	Intracellular Staining by Flow Cytometry: 10 µL/10⁶cells
来源	The product is shipped with polar packs. Upon receipt, store it immediately at the temperature recommended below.
产品组分

性能
供应商	R&D Systems
Entrez Gene IDs	3592 (Human); 16159 (Mouse); 84405 (Rat)
应用文献
R&D Systems personnel manually curate a database that contains references using R&D Systems products. The data collected includes not only links to publications in PubMed, but also provides information about sample types, species, and experimental conditions. B Lymphocytes in Multiple Sclerosis: Bregs and BTLA/CD272 Expressing-CD19+ Lymphocytes Modulate Disease Severity Sci Rep, 2016;6(0):29699. Species: Human Sample Type: Whole Cells Application: Flow A novel data mining system points out hidden relationships between immunological markers in multiple sclerosis. Authors: Gironi, Maira, Saresella, Marina, Rovaris, Marco, Vaghi, Matilde, Nemni, Raffaell, Clerici, Mario, Grossi, Enzo Immun Ageing, 2013;10(1):1. Species: Human Sample Type: whole cells Application: Flow Vitamin D receptor gene polymorphisms and HLA DRB1*04 cosegregation in Saudi type 2 diabetes patients. Authors: Al-Daghri NM, Al-Attas O, Alokail MS J. Immunol., 2012;188(3):1325-32. Species: Human Sample Type: Whole Cells Application: Flow A novel small molecule inhibitor of signal transducers and activators of transcription 3 reverses immune tolerance in malignant glioma patients. Authors: Hussain SF, Kong LY, Jordan J, Conrad C, Madden T, Fokt I, Priebe W, Heimberger AB Cancer Res., 2007;67(20):9630-6. Species: Human Sample Type: Whole Cells Application: Flow Nucleoporation of dendritic cells: efficient gene transfer by electroporation into human monocyte-derived dendritic cells. Authors: Lenz P, Bacot SM, Frazier-Jessen MR, Feldman GM FEBS Lett., 2003;538(1):149-54. Species: Human Sample Type: Whole Cells Application: Flow
纯化方式	Protein A or G purified from ascites
免疫原	S. frugiperda insect ovarian cell line Sf 21-derived recombinant human IL‑12/IL-35 p35 Arg23-Ser219 Accession # P29459
生物活性	Human, Mouse
标记	Fluorescein
背景	Interleukin12 (IL-12) and interleukin 35 (IL-35) are heterodimeric cytokines composed of alpha and beta chains. IL-12 is composed of p35 and p40 subunits, while IL-35 iscomprised of p35 paired with EBI-3 (1). In mice, IL-35 is produced by FoxP3⁺regulatory T cells and may function as an inhibitory cytokine to suppress Tcell proliferation (2). Human FoxP3⁺ Tregs do not constitutivelyexpress IL-35 (3), but expression may be induced by activated dendritic cells(4).
运输条件	Blue Ice
存放说明	4℃
参考文献	Collison, L.W. and D.A.A. Vignali (2008) Immunol. Rev. 226:248. Collison, L.W. et al. (2007) Nature 450:566. Bardel,E.et al. (2008) J. Immunol. 181:6898. Seyerl, M. et al.(2010) Eur. J. Immunol. 40:321.

参考图片

Detection of IL‑12/IL‑35 p35 in Human PBMCs by Flow Cytometry. Human peripheral blood mononuclear cells (PBMCs) either untreated (light orange filled histogram) or treated with LPS (dark orange filled histogram) were stained with Mouse Anti-Human/Mouse IL‑12/IL‑35 p35 Fluorescein‑conjugated Monoclonal Antibody (Catalog # IC2191F) or isotype control antibody (Catalog # IC002F, open histogram). To facilitate intracellular staining, cells were fixed with Flow Cytometry Fixation Buffer (Catalog # FC004) and permeabilized with Flow Cytometry Permeabilization/Wash Buffer I (Catalog # FC005). View our protocol for Staining Intracellular Molecules.

Detection of IL‑12/IL‑35 p35 in Mouse Splenocytes by Flow Cytometry. Mouse splenocytes either untreated (light orange filled histogram) or treated with LPS (dark orange filled histogram) were stained with Mouse Anti-Human/Mouse IL‑12/IL‑35 p35 Fluorescein‑conjugated Monoclonal Antibody (Catalog # IC2191F) or isotype control antibody (Catalog # IC002F, open histogram). To facilitate intracellular staining, cells were fixed with Flow Cytometry Fixation Buffer (Catalog # FC004) and permeabilized with Flow Cytometry Permeabilization/Wash Buffer I (Catalog # FC005). View our protocol for Staining Intracellular Molecules.