| 货号 | IC2506F |
| 别名 | EC 2.7.11; EC 2.7.11.26; glycogen synthase kinase 3 beta; glycogen synthase kinase-3 beta; GSK-3 beta; GSK3B; GSK3beta isoform | 全称 | Glycogen Synthase Kinase 3 beta |
| 反应种属 | Human |
| 应用 | Intracellular Staining by Flow Cytometry(10 µL/106cells) |
| 目标/特异性 | Detects human, mouse, and rat GSK-3 beta in ELISAs and Western blots. Does not cross-react with recombinant human GSK-3 alpha. |
| 使用方法 | Intracellular Staining by Flow Cytometry: 10 µL/106cells |
| 来源 | The product is shipped with polar packs. Upon receipt, store it immediately at the temperature recommended below. |
| 产品组分 |
| 供应商 | R&D Systems |
| Entrez Gene IDs | 2932 (Human); 56637 (Mouse); 84027 (Rat) |
| 纯化方式 | Protein A or G purified from hybridoma culture supernatant |
| 免疫原 | E. coli-derived recombinant human GSK-3 beta Met1-Thr420 Accession # P49841 |
| 生物活性 | Human, Mouse, Rat |
| 标记 | Fluorescein |
| 背景 | Glycogen Synthase Kinase-3 (GSK-3) is a serine/threonine kinase initially identified as an inhibitor of glycogen synthase. Two isoforms (GSK-3 alpha and GSK-3 beta ) share 85% amino acid sequence identity. GSK-3 beta, inhibited by phosphorylation at S9 by Akt, is involved in energy metabolism, body pattern formation, and neuronal cell development. Depending upon the substrate, phosphorylation may either promote activity (SIK-1; OGT; ZC3HAV1) or downregulate activity (SNAI1; BMAL1; MARK2). |
| 运输条件 | Blue Ice |
| 存放说明 | 4℃ |
| 参考文献 |
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Detection of GSK‑3 beta in HeLa Human Cell Line by Flow Cytometry. HeLa human cervical epithelial carcinoma cell line was stained with Rat Anti-Human/Mouse/Rat GSK‑3 beta Fluorescein‑conjugated Monoclonal Antibody (Catalog # IC2506F, filled histogram) or isotype control antibody (Catalog # IC006F, open histogram). To facilitate intracellular staining, cells were fixed with Flow Cytometry Fixation Buffer (Catalog # FC004) and permeabilized with Flow Cytometry Permeabilization/Wash Buffer I (Catalog # FC005). View our protocol for Staining Intracellular Molecules. |
