| 货号 | IC1834F |
| 别名 | IL-27 p28 subunit; IL-27 subunit alpha; IL-27; IL27A; IL-27-A; IL27-A; IL27p28; IL30; interleukin 27; interleukin 30; interleukin-27 subunit alpha; MGC71873; p28IL-27A | 全称 | Interleukin 27 |
| 反应种属 | Mouse |
| 应用 | Intracellular Staining by Flow Cytometry(10 µL/106cells) |
| 目标/特异性 | Detects mouse IL-27 in Western blots. This antibody recognizes the p28 subunit either as part of a p28/EBI-3 heterodimer or as a free subunit. |
| 使用方法 | Intracellular Staining by Flow Cytometry: 10 µL/106cells |
| 来源 | The product is shipped with polar packs. Upon receipt, store it immediately at the temperature recommended below. |
| 产品组分 |
| 供应商 | R&D Systems |
| Entrez Gene IDs | 246778 (Human); 246779 (Mouse) |
| 应用文献 | |
| R&D Systems personnel manually curate a database that contains references using R&D Systems products. The data collected includes not only links to publications in PubMed, but also provides information about sample types, species, and experimental conditions. The transcription factor IRF8 activates integrin-mediated TGF-beta signaling and promotes neuroinflammation. | |
| 纯化方式 | Protein A or G purified from hybridoma culture supernatant |
| 免疫原 | Chinese hamster ovary cell line CHO-derived recombinant mouse IL‑27 Tyr19-Pro228 (EBI3) & Phe29-Ser234 (p28) Accession # O35228 (EBI3) & Q8K3I6 (p28) |
| 生物活性 | Mouse |
| 标记 | Fluorescein |
| 背景 | IL-27 is anon-covalent heterodimeric molecule that belongs to the IL-6/IL-12 family oflong type I cytokines. It is composed of EBI3 (EBV-induced gene 3), a33-34 kDa glycoprotein that is related to the p40 subunit of IL-12 and IL-23,and p28, the 28-30 kDa glycoprotein that is related to the p35 chain ofIL-12. The mouse EBI3 gene encodes a 228 amino acid (aa) precursor thatcontains an 18 aa signal peptide and 210 aa mature protein. The mature regioncontains two potential N-linked glycosylation sites, two fibronectin type IIIdomains, and two pairs of conserved cysteine residues with a WSXWS-like motifthat places the molecule in the hematopoietin receptor family. Although p40,the EBI3 counterpart in IL-12, is known to form homodimers, there is no evidenceto date that EBI3 also homodimerizes. EBI3 is known to heterodimerize with theIL-12 p35 subunit to form IL-35. Mature mouse EBI3 shares 91% and 61% aasequence identity to mature rat and human EBI3, respectfully. The mouse p28gene encodes a 234 aa precursor that contains a 28 aa signal sequence and 206 aamature region. The mature region is characterized by the presence of four alpha -helices, placing it in the IL-6 family of helical cytokines. Mature mouse p28shares 89% and 70% aa sequence identity with mature rat and human p28,respectfully. IL-27 is expressed by monocytes, endothelial cells and dendriticcells. IL-27 binds to and signals through a heterodimeric receptor complexcomposed of WSX-1 (TCCR) and gp130. Evidence suggests IL-27 interacts only with WSX-1. In addition, and inmouse but not human, p28 is secreted as a monomer and appears to activate theIL-6R complex. This is consistent with the observation that p28 alsoheterodimerizes with CLF1, and signals through the IL-6R:gp130complex. IL-27 has both anti- and proinflammatory properties. As an anti‑inflammatory,IL-27 seems to induce a general negative feedback program that limits T andNK-T cell activity. At the onset of infection, IL-27 induces an IL‑12 receptoron naïve CD4+ T cells, making them susceptible to subsequent IL-12activity that generates Th1 cells at the expense of Th2 and Th17 cells.Finally, IL-27 upregulates both MHC-II and chemokine (CXCL9; CXCL10) expressionon vascular endothelium, suggesting a role for IL-27 in vascular inflammation. |
| 运输条件 | Blue Ice |
| 存放说明 | 4℃ |
| 参考文献 |
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Detection of IL‑27 in RAW 264.7 Mouse Cell Line by Flow Cytometry. RAW 264.7 mouse monocyte/macrophage cell line was stained with Rat Anti-Mouse IL‑27 Fluorescein‑conjugated Monoclonal Antibody (Catalog # IC1834F, filled histogram) or isotype control antibody (Catalog # IC013F, open histogram). To facilitate intracellular staining, cells were fixed with Flow Cytometry Fixation Buffer (Catalog # FC004) and permeabilized with Flow Cytometry Permeabilization/Wash Buffer I (Catalog # FC005). View our protocol for Staining Intracellular Molecules. |
