| 货号 | FAB983P |
| 别名 | CD123 antigen; CD123; hIL3Ra; hIL-3Ra; IL-3 receptor subunit alpha; IL-3R subunit alpha; IL3R; IL3RA; IL-3RA; IL-3R-alpha; IL3RAY; IL3RX; IL3RY; interleukin 3 receptor, alpha (low affinity); interleukin-3 receptor subunit alpha; MGC34174 | 全称 | Interleukin 3 Receptor alpha |
| 反应种属 | Mouse |
| 应用 | Flow Cytometry |
| 目标/特异性 | Detects mouse IL-3 R alpha /CD123 in direct ELISAs and Western blots. In direct ELISAs and Western blots, no cross-reactivity with recombinant human IL-3 R alpha and recombinant mouse IL-3 R beta is observed. |
| 使用方法 | Flow Cytometry: 10 µL/106cells |
| 来源 | The product is shipped with polar packs. Upon receipt, store it immediately at the temperature recommended below. |
| 产品组分 |
| 供应商 | R&D Systems |
| Entrez Gene IDs | 3563 (Human); 16188 (Mouse) |
| 纯化方式 | Protein A or G purified from hybridoma culture supernatant |
| 免疫原 | S. frugiperda insect ovarian cell line Sf 21-derived recombinant mouse IL-3 R alpha /CD123 Ser17-Lys331 Accession # P26952 |
| 生物活性 | Mouse |
| 标记 | Phycoerythrin |
| 背景 | Interleukin 3 (IL-3) is a cytokine produced primarily by activated T cells or mast cells. IL-3 stimulates the proliferation and differentiation of hematopoietic cells including pluripotent hematopoietic stem cells as well as various lineage-committed cells. The biological effects of IL-3 on the various cell types are mediated by the binding of IL-3 to a specific cell surface receptor complex. The functional high-affinity IL-3 receptor is a heterodimer consisting of a ligand binding alpha subunit and a beta subunit. The alpha subunit alone binds IL-3 with low affinity. The beta subunit is required for the high-affinity binding of IL-3 to the heterodimeric receptor complex. The beta subunit has also been found to be a component of the high-affinity receptor complex for IL-5 and GM-CSF and is also referred to as the beta common ( beta c) chain. In the mouse, there are two IL-3 R beta proteins. The first identified mouse IL-3 R beta was also called AIC2A and binds IL-3 with low affinity (1). The second mIL-3 R beta was referred to as AIC2B (2). AIC2B doesn’t bind IL-3 and is the homolog of the human IL-3 R beta. AIC2A was found to be the result of a gene duplication event. The mouse IL-3 R alpha, also called SUT-1, will form complexes with either mouse IL-3 R beta protein (3). The alpha subunit is a member of the type I cytokine receptor family, type 5 subfamily (4,5). The mouse IL-3 R alpha extracellular domain (ECD) shares 42% and 29% amino acid sequence identity with rat and human IL-3 R alpha ECD, respectively. |
| 运输条件 | Blue Ice |
| 存放说明 | 4℃ |
| 参考文献 |
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Detection of IL‑3 R alpha /CD123 in IL-3 Dependent DA3 Mouse Cell Line by Flow Cytometry. IL-3 dependent myeloid leukemia DA3 mouse myeloma cell line was stained with Rat Anti-Mouse IL‑3 R alpha /CD123 PE‑conjugated Monoclonal Antibody (Catalog # FAB983P, filled histogram) or isotype control antibody (Catalog # IC006P, open histogram). View our protocol for Staining Membrane-associated Proteins. |
