Mouse IL-1 beta /IL-1F2 Fluorescein-conjugated Antibody【科瑞奥博】

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Mouse IL-1 beta /IL-1F2 Fluorescein-conjugated Antibody

货号： IC4013F 基本售价： 3390.7 元规格： 100 Tests

产品信息

概述
货号	IC4013F
别名	catabolin; IL1 beta; IL-1 beta; IL-1; IL1B; IL-1B; IL1-BETA; IL-1F2; IL1F2IL-1 beta; interleukin 1, beta; interleukin-1 beta; preinterleukin 1 beta; pro-interleukin-1-beta
全称	Interleukin 1 beta
应用	Intracellular Staining by Flow Cytometry
目标/特异性	Detects mouse IL-1 beta /IL-1F2 in direct ELISAs. In direct ELISAs, approximately 75% cross-reactivity with recombinant guinea pig IL-1 beta, 5‑15% cross-reactivitiy with recombinant canine, cotton rat, feline, equine, and rat IL-1 beta, and no cross-reactivity with recombinant human, porcine, and rhesus IL-1 beta is observed. No cross-reactivity with recombinant human (rh) Pro-IL-1 beta, rhIL-1F7, rhIL-1F10, rhIL-36 gamma, recombinant mouse (rm) IL-1 alpha, rmIL-18, rmIL-36Ra, rmIL-36 alpha, or rmIL-36 beta is observed.
使用方法	Intracellular Staining by Flow Cytometry: 10 µL/10⁶cells
来源	The product is shipped with polar packs. Upon receipt, store it immediately at the temperature recommended below.
产品组分

性能
供应商	R&D Systems
Entrez Gene IDs	3553 (Human); 16176 (Mouse); 24494 (Rat); 397122 (Porcine); 403974 (Canine); 100034237 (Equine); 100135556 (Guinea Pig)
纯化方式	Protein A or G purified from hybridoma culture supernatant
免疫原	E. coli-derived recombinant mouse IL-1 beta /IL-1F2 Val118-Ser269 Accession # P10749
生物活性	Mouse
标记	Fluorescein
背景	IL-1 beta is a pro-inflammatory cytokine belonging to the IL-1 superfamily, designated IL-1F2. IL-1 beta and IL-1 alpha /IL-1F1 bind the same receptors but vary in expression and affinity. They share only 25% amino acid (aa) sequence identity and are produced from separate genes. IL-1 beta is synthesized as a 31 kDa pro-form that is cleaved intracellularly by caspase-1 to produce the mature active 17.5 kDa IL-1 beta. Mature mouse IL-1 beta shares 74% and 91% aa identity with human and rat IL-1 beta, respectively.
运输条件	Blue Ice
存放说明	4℃
参考文献	Cancer Biomarkers Conventional/Classical Dendritic Cells Cytokines and Receptors in Allergy and Asthma Cytokines and Receptors in Angiogenesis IL-1 Family Infectious Diseases Inflammatory Mediators Isolation, Culture, and Identification of Natural Killer Cells LTi Cells Macrophage Activation Markers Multiple Sclerosis and Myelin Related Molecules Myeloid-derived Suppressor Cells (MDSC) NCR-ILC3 Neuroinflammation NOD-like Receptors and the Inflammasome Platelets Regulatory T Cells (Tregs) T Cell Cytokine Signaling Th17 Cells Th9 Cells

参考图片

Detection of IL‑1 beta /IL‑1F2 in Mouse Splenocytes by Flow Cytometry. Mouse splenocytes, untreated (open histogram), or treated with LPS for 48 hours (filled histogram) were stained with Rat Anti-Mouse IL‑1 beta /IL‑1F2 Fluorescein‑conjugated Monoclonal Antibody (Catalog # IC4013F, filled histogram) or isotype control antibody (Catalog # IC013F, data not shown). Histograms were gated on blasting splenocytes. To facilitate intracellular staining, cells were fixed with Flow Cytometry Fixation Buffer (Catalog # FC004) and permeabilized with Flow Cytometry Permeabilization/Wash Buffer I (Catalog # FC005). View our protocol for Staining Intracellular Molecules.