货号 | FAB566F |
别名 | BDCA4; BDCA-4; CD304 antigen; CD304; DKFZp686A03134; DKFZp781F1414; neuropilin 1; neuropilin-1; NRP1; NRPNP1; transmembrane receptor; Vascular endothelial cell growth factor 165 receptor; VEGF165RCD304 |
反应种属 | Mouse/Rat |
应用 | Flow Cytometry(10 µL/106cells) |
目标/特异性 | Detects mouse and rat Neuropilin-1 in direct ELISAs and Western blots. In direct ELISAs, approximately 8% cross-reactivity with recombinant human Neuropilin‑1 is observed, and less than 1% cross-reactivity with recombinant rat Neuropilin-2 is observed. |
使用方法 | Flow Cytometry: 10 µL/106cells |
来源 | The product is shipped with polar packs. Upon receipt, store it immediately at the temperature recommended below. |
产品组分 |
供应商 | R&D Systems |
Entrez Gene IDs | 8829 (Human); 18186 (Mouse); 246331 (Rat) |
纯化方式 | Antigen Affinity-purified |
免疫原 | Mouse myeloma cell line NS0-derived recombinant rat Neuropilin‑1 Phe22-Ala810 (Lys811Arg), Ser829-Asp854 Accession # Q9QWJ9 |
生物活性 | Mouse, Rat |
标记 | Fluorescein |
背景 | Neuropilin-1 is a type I transmembrane protein that is expressed in the developing nervous system and by endothelial and tumor cells. Neuropilin-1 binds members of the class III secreted semaphorin subfamily as well as some isoforms of VEGF family proteins. The amino acid sequence of rat Neuropilin-1 extracellular domain is 98% and 93% identical to that of mouse and human Neuropilin-1, respectively. |
运输条件 | Blue Ice |
存放说明 | 4℃ |
参考文献 |
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Detection of Neuropilin‑1 in bEnd.3 Mouse Cell Line by Flow Cytometry. bEnd.3 mouse endothelioma cell line was stained with Goat Anti-Mouse/Rat Neuropilin‑1 Fluorescein‑conjugated Antigen Affinity-purified Polyclonal Antibody (Catalog # FAB566F, filled histogram) or isotype control antibody (Catalog # IC108F, open histogram). View our protocol for Staining Membrane-associated Proteins. | |
Detection of Neuropilin‑1 in PC‑12 Rat Cell Line by Flow Cytometry. PC‑12 rat adrenal pheochromocytoma cell line was stained with Goat Anti-Mouse/Rat Neuropilin‑1 Fluorescein‑conjugated Antigen Affinity-purified Polyclonal Antibody (Catalog # FAB566F, filled histogram) or isotype control antibody (Catalog # IC108F, open histogram). View our protocol for Staining Membrane-associated Proteins. |