| 货号 | FAB2607F |
| 简介 | FAB2607F has been discontinued.View all Glypican 5 products. |
| 别名 | bA93M14.1; glypican 5; glypican proteoglycan 5; glypican-5; GPC5 |
| 反应种属 | Human/Mouse |
| 应用 | Flow Cytometry(10 µL/106cells) |
| 目标/特异性 | Detects human and mouse Glypican 5 in direct ELISAs and Western blots. In direct ELISAs and Western blots, 100% cross‑reactivity with recombinant mouse Glypican 5 is observed and no cross‑reactivity with recombinant human Glypican 2, 3 or 6 is observed. |
| 使用方法 | Flow Cytometry: 10 µL/106cells |
| 来源 | The product is shipped with polar packs. Upon receipt, store it immediately at the temperature recommended below. |
| 产品组分 |
| 供应商 | R&D Systems |
| Entrez Gene IDs | 2262 (Human); 103978 (Mouse) |
| 纯化方式 | Protein A or G purified from hybridoma culture supernatant |
| 免疫原 | Mouse myeloma cell line NS0-derived recombinant human Glypican 5 Glu25-Thr554 Accession # P78333 |
| 生物活性 | Human, Mouse |
| 标记 | Fluorescein |
| 背景 | Glypican 5 (GPC5) belongs to the Glypican family of heparan sulfate proteoglycansthat are linked to the cell surface through aglycosyl-phosphatidylinositol anchor. It is synthesized as a glycosylated core protein of 63-65 kDa size that is further modified by both heparan and chondroitin sulfate adducts to create a 150-170 kDa glycosaminoglycan (GAG). Glypican 5 is principally expressed in neurons or neuronal precursors. As such, one role for Glypican 5 is to act as a co-receptor for SHH where it promotes SHH:Patched/PTC-1 interaction through its sulfated GAGs. Over amino acids 25-554, human and mouse Glypican 5 share 87% amino acid sequence identity. |
| 运输条件 | Blue Ice |
| 存放说明 | 4℃ |
| 参考文献 |
|
Detection of Glypican 5 in H9 Human Cell Line by Flow Cytometry. H9 human cutaneous T lymphoma cell line was stained with Mouse Anti-Human/Mouse Glypican 5 Fluorescein‑conjugated Monoclonal Antibody (Catalog # FAB2607F, filled histogram) or isotype control antibody (Catalog # IC003F, open histogram). View our protocol for Staining Membrane-associated Proteins. |
