| 货号 | FAB796P |
| 别名 | BB2; CD54 antigen; CD54; cell surface glycoprotein P3.58; human rhinovirus receptor; ICAM-1; intercellular adhesion molecule 1 (CD54), human rhinovirus receptor; intercellular adhesion molecule 1; Major group rhinovirus receptor; P3.58 | 全称 | Intercellular Adhesion Molecule 1 |
| 反应种属 | Mouse |
| 应用 | Flow Cytometry |
| 目标/特异性 | Detects mouse ICAM-1 in ELISAs and Western blots. In Western blots, this antibody shows weak cross-reactivity with recombinant human (rh) ICAM‑1 and recombinant mouse (rm) ICAM-2 and no cross-reactivity with rhCD31, rmDCC, rhICAM-2, rhICAM-3, recombinant rat (rr) ICAM-1, rmMAdCAM-1, or rmVCAM-1. |
| 使用方法 | Flow Cytometry: 10 µL/106cells |
| 来源 | The product is shipped with polar packs. Upon receipt, store it immediately at the temperature recommended below. |
| 产品组分 |
| 供应商 | R&D Systems |
| Entrez Gene IDs | 3383 (Human); 15894 (Mouse); 25464 (Rat) |
| 应用文献 | |
| R&D Systems personnel manually curate a database that contains references using R&D Systems products. The data collected includes not only links to publications in PubMed, but also provides information about sample types, species, and experimental conditions. ICAM-1-based rabies virus vaccine shows increased infection and activation of primary murine B cells in vitro and enhanced antibody titers in-vivo. | |
| 纯化方式 | Protein A or G purified from hybridoma culture supernatant |
| 免疫原 | Mouse myeloma cell line NS0-derived recombinant mouse ICAM-1 Gln28-Asn485 Accession # P13597 |
| 生物活性 | Mouse |
| 标记 | Phycoerythrin |
| 背景 | ICAM-1 (InterCellular Adhesion Molecule-1), also known as CD54, is an85-93 kDa member of the Immunoglobulin supergene family of molecules. Mouse ICAM-1 is a 510 amino acid (aa) type Itransmembrane glycoprotein that contains five C2-type Ig-like domains in a 458aa extracellular domain (ECD) (aa 28-485). It is expressed constitutively by, or inducibly on, a very wide variety ofcells, including neurons, dendritic cells, fibroblasts, skeletal musclemyocytes, keratinocytes, eosinophils, neutrophils, hepatocytes, monocytes andvascular endothelial cells. On the cellsurface, it exists primarily as an "intermediate" between a homodimeric andmonomeric state. The homodimeric pairingdoes not seem to offer a distinct advantage with respect to ligandbinding. On the other hand, the degreeof glycosylation has a marked impact on ICAM-1 ligand binding, with under-glycosylationassociated with low affinity ligand binding. Ligands for ICAM-1 include LFA-1 ( alpha L beta 2), Mac-1 ( alpha M beta 2), CD11c/CD18, fibrinogen,hyaluronan and select rhinoviruses. Notably, ICAM-1 is recognized to be a potential coreceptor for HGF,acting in concert with c-Met during ligand binding. ICAM-1 is best known for its role in whitecell trafficking (leukocyte binding to endothelium), and is also reported toplay a key role in the establishment of CD8+ T cell memory, serving as a linkbetween mature dendritic cells and naïve CD8+ T cells. Over its ECD, mouse ICAM-1 shares 78% and 54%aa sequence identity with rat and human ICAM-1 ECD, respectively. There is one isoform variant for mouse ICAM-1listed in SwissProt that shows a 28 aa substitution for aa 1-34. |
| 运输条件 | Blue Ice |
| 存放说明 | 4℃ |
| 参考文献 |
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Detection of ICAM‑1/CD54 in Mouse Splenocytes by Flow Cytometry. Mouse splenocytes were stained with Rat Anti-Mouse ICAM‑1/CD54 PE‑conjugated Monoclonal Antibody (Catalog # FAB796P, filled histogram) or isotype control antibody (Catalog # IC013P, open histogram). View our protocol for Staining Membrane-associated Proteins. |
