| 货号 | FAB11201F |
| 别名 | DKFZp564G072; FLJ95923; frizzled family receptor 1; frizzled homolog 1; frizzled, Drosophila, homolog of, 1; frizzled-1; fz-1; FZD1; fzE1; hFz1; seven transmembrane spanning receptor; Wnt receptor |
| 反应种属 | Human/Mouse |
| 应用 | Flow Cytometry |
| 目标/特异性 | Detects human and mouse Frizzled-1 in direct ELISAs and Western blots. In direct ELISAs, approximately 30% cross‑reactivity with recombinant mouse (rm) Frizzled-9 is observed and no cross-reactivity with recombinant human (rh) Frizzled-5, rm Frizzled 2, 3, 4, 6, 7, 8, or rhMFRP is observed. |
| 使用方法 | Flow Cytometry: 10 µL/106cells |
| 来源 | The product is shipped with polar packs. Upon receipt, store it immediately at the temperature recommended below. |
| 产品组分 |
| 供应商 | R&D Systems |
| Entrez Gene IDs | 8321 (Human); 14362 (Mouse) |
| 纯化方式 | Protein A or G purified from hybridoma culture supernatant |
| 免疫原 | Mouse myeloma cell line NS0-derived recombinant mouse Frizzled‑1 Gln1-His248 (Met122Ile) Accession # O70421 |
| 生物活性 | Human, Mouse |
| 标记 | Fluorescein |
| 背景 | Members of the Fz/Smo family of proteins serve as receptors in the Wnt signaling pathway and are involved in a variety of developmental processes. Frizzled-1 (Fzd-1) is a 75-80 kDa 7-transmembrane glycoprotein found on chondrocytes and in the presynaptic membranes of neurons. It is also reported to be found on fibroblasts, osteoblasts, enothelium and vascular smooth muscle, and in the embryo in mesoderm. Fzd-1 interacts with LRP-5 to generate a receptor complex for Wnts. Wnts known to bind Fzd-1 include Wnt-1, -2, -3, -3a, and -8. Over amino acids (aa) 1-248, human and mouse Fzd-1 share 87% aa sequence identity. |
| 运输条件 | Blue Ice |
| 存放说明 | 4℃ |
| 参考文献 |
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Detection of Frizzled‑1 in HEK293 Human Cell Line by Flow Cytometry. HEK293 human embryonic kidney cell line was stained with Rat Anti-Human/Mouse Frizzled‑1 Fluorescein‑conjugated Monoclonal Antibody (Catalog # FAB11201F, filled histogram) or isotype control antibody (Catalog # IC006F, open histogram). View our protocol for Staining Membrane-associated Proteins. | |
Detection of Frizzled‑1 in D3 Mouse Cell Line treated with Retinoic Acid by Flow Cytometry. D3 mouse embryonic stem cell line treated with retinoic acid was stained with Rat Anti-Human/Mouse Frizzled‑1 Fluorescein‑conjugated Monoclonal Antibody (Catalog # FAB11201F, filled histogram) or isotype control antibody (Catalog # IC006F, open histogram). View our protocol for Staining Membrane-associated Proteins. |
