Mouse MARCO Fluorescein MAb (Clone 579511) (100 TESTS)【科瑞奥博】

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Mouse MARCO Fluorescein MAb (Clone 579511) (100 TESTS)

货号： FAB2956F 基本售价： 3242.0 元规格： 100 Tests

产品信息

概述
货号	FAB2956F
别名	macrophage receptor with collagenous structureSCARA2Scavenger receptor class A member 2; member 2; SCARA2
全称	Macrophage Receptor with Collagenous Structure/Scavenger Receptor, Class A, Member 2
反应种属	Mouse
应用	Flow Cytometry(10 µL/10⁶cells)
目标/特异性	Detects mouse MARCO in direct ELISAs.
使用方法	Flow Cytometry: 10 µL/10⁶cells
来源	The product is shipped with polar packs. Upon receipt, store it immediately at the temperature recommended below.
产品组分

性能
供应商	R&D Systems
Entrez Gene IDs	8685 (Human); 17167 (Mouse)
纯化方式	Protein A or G purified from hybridoma culture supernatant
免疫原	Mouse myeloma cell line NS0-derived recombinant mouse MARCO Gln70-Ser518 Accession # Q60754
生物活性	Mouse
标记	Fluorescein
背景	MARCO (macrophage receptor with collagenous structure), also known as SCARA2, is an 80 kDa type II transmembrane glycoprotein that belongs to the class A scavenger receptor family (1). Mouse MARCO consists of a 48 amino acid (aa) cytoplasmic domain, a 21 aa transmembrane segment, and a 449 aa extracellular domain (ECD) that includes a stalk region, a collagen-like region, and one SRCR domain (2). Within the ECD, mouse MARCO shares 69% and 86% aa sequence identity with human and rat MARCO, respectively. It shares 18%‑28% aa sequence identity with other mouse class A scavenger receptors CL-P1, SCARA3, SCARA5, and SR-A1/MSR. MARCO is constitutively expressed on the surface of splenic and lymph node macrophages (2, 3). Its expression is induced on Kupffer cells and alveolar macrophages by microbial infection, chemical irritants, and Th1 polarizing factors (3‑5). MARCO binds LPS, lipoteichoic acid, and other determinants on Gram positive and Gram negative bacteria (2, 6‑8). It also binds modified LDL, CpG oligonucleotides, UGRP1, silica, and TiO₂ (2, 9‑11). MARCO is required for the organization of the splenic marginal zone and the interaction of local macrophages and B cells (12, 13). The SRCR domain mediates binding of MARCO to its various ligands (3, 12), while the collagen-like region mediates assembly into a disulfide-linked trimeric molecule (2, 7). MARCO ligation induces, but is not required for the production of IL-12, NO, or TNF-alpha by macrophages (5, 6, 9). MARCO knockout mice show a reduced clearance of bacterial infections, reduced mast cell mediated silicosis, increased pulmonary inflammation, and increased sensitivity to ozone induced lung damage (4, 9, 14‑16).
运输条件	Blue Ice
存放说明	4℃
参考文献	Murphy, J.E. et al. (2005) Atherosclerosis 182:1. Elomaa, O. et al. (1995) Cell 80:603. Van der Laan, L.J.W. et al. (1999) J. Immunol. 162:939. Dahl, M. et al. (2007) J. Clin. Invest. 117:757. Jozefowski, S. et al. (2005) J. Immunol. 175:8032. Mukhopadhyay, S. et al. (2006) Eur. J. Immunol. 36:940. Sankala, M. et al. (2002) J. Biol. Chem. 277:33378. Chen, Y. et al. (2006) J. Biol. Chem. 281:12767. Jozefowski, S. et al. (2006) J. Leukoc. Biol. 80:870. Bin, L.-H. et al. (2003) J. Immunol. 171:924. Hamilton, Jr. R.F. et al. (2006) J. Biol. Chem. 281:34218. Karlsson, M.C.I. et al. (2003) J. Exp. Med. 198:333. Chen, Y. et al. (2005) J. Immunol. 175:8173. Arredouani, M. et al. (2004) J. Exp. Med. 200:267. Arredouani, M.S. et al. (2007) J. Immunol. 178:5912. Brown, J.M. et al. (2007) Am. J. Respir. Cell Mol. Biol. 36:43.

参考图片

Detection of MARCO in Mouse Splenocytes by Flow Cytometry. Mouse splenocytes were stained with Rat Anti-Mouse MARCO Fluorescein-conjugated Monoclonal Antibody (Catalog # FAB2956F, filled histogram) or isotype control antibody (Catalog # IC005F, open histogram). View our protocol for Staining Membrane-associated Proteins.