Mouse CD39/ENTPD1 Fluorescein MAb (Clone 495826) (100 TESTS)【科瑞奥博】

产品中心

当前位置：首页>产品中心

Mouse CD39/ENTPD1 Fluorescein MAb (Clone 495826) (100 TESTS)

货号： FAB4398F 基本售价： 2676.9 元规格： 100 Tests

产品信息

概述
货号	FAB4398F
别名	ADPase; ATPDase; ATP-diphosphatase; CD39 antigen; CD39; CD39EC 3.6.1.5; DKFZp686D194; DKFZp686I093; EC 3.6.1; ecto-apyrase; Ecto-ATP diphosphohydrolase 1; Ecto-ATPase 1; Ecto-ATPDase 1; ectonucleoside triphosphate diphosphohydrolase 1; ENTPD1; FLJ40921; FLJ40959; Lymphoid cell activation antigen; NTPD1; NTPDase 1; NTPDase-1
全称	Ecto-Nucleoside Triphosphate Diphosphohydrolase 1
反应种属	Mouse
应用	Flow Cytometry(10 µL/10⁶cells)
目标/特异性	Detects mouse CD39/ENTPD1 in direct ELISAs and Western blots. In direct ELISAs, approximately 15% cross-reactivity with recombinant human CD39 is observed and no cross-reactivity with recombinant mouse CD39L3 or recombinant human CD39L4 is observed.
使用方法	Flow Cytometry: 10 µL/10⁶cells
来源	The product is shipped with polar packs. Upon receipt, store it immediately at the temperature recommended below.
产品组分

性能
供应商	R&D Systems
Entrez Gene IDs	953 (Human); 12495 (Mouse); 64519 (Rat)
纯化方式	Protein A or G purified from hybridoma culture supernatant
免疫原	Mouse myeloma cell line NS0-derived recombinant mouse CD39/ENTPD1 Thr38-Ile478 Accession # AAH11278
生物活性	Mouse
标记	Fluorescein
背景	Ectonucleoside Triphosphate Diphosphohydrolase-1 (ENTPD1) is a 65-80 kDa, two-transmembrane glycoprotein that contains an N- and C-terminal cytoplasmic domain (1, 2). ENTPD1 was originally described as CD39, a B lymphocyte cell surface marker and is now known to also be present on the surface of endothelial cells, neutrophils, mast cells, macrophages, pancreatic acinar and duct cells, dendritic cells, sympathetic neuron axon terminals, CD4⁺CD39⁺CD161⁺ Th17 precursors, PD-1⁺CD39⁺ "exhausted" CD8⁺ T cells and FoxP3⁺CD25⁺CD4⁺CD127^-CD49d^- suppressor plus FoxP3^-CD25^-CD4⁺CD127⁺CD49d⁺ non-suppressor T cells (1-9). ENTPD1 hydrolyzes the beta - and gamma phosphate residues of nucleotides, preferring ATP as the substrate. Through its hydrolysis of extracellular nucleotides, ENTPD1 plays a role in the regulation of purinergic signaling. Extracellular ATP released from dead or stressed cell creates a proinflammatory environment. In concert with CD73, the conversion of ATP to adenosine reverses this and creates an antiinflammatory environment (1,2). Over amino acids (aa) 38-478, mouse ENTPD1 shares 90% and 76% aa sequence identity with rat and human ENTPD1, respectively
运输条件	Blue Ice
存放说明	4℃
参考文献	Bono, M.R. et al. (2015) FEBS Lett. 589:3454. Antonioli, L. et al. (2013) Trends Mol. Med. 19:355. Machida, T. et al. (2005) J. Pharmacol. Exp. Ther. 313:570. Aldi, S. et al. (2015) FASEB J. 29:61. Schuler, P.J. et al. (2011) J. Immunol. Methods 369:59. Gupta, P.K. et al. (2015) PLoS Pathog. 11:e1005177. Bai, A. and S. Robson (2015) Purinergic Signal. 11:317. Sorenson, C.E. et al. (2003) J. Physiol. 551:881. Mascanfroni, I.D. et al. (2013) Nat. Immunol. 14:1054.

参考图片

Detection of CD39/ENTPD1 in Mouse Splenocytes by Flow Cytometry. Mouse splenocytes were stained with Rat Anti-Mouse CD39/ENTPD1 Fluorescein‑conjugated Monoclonal Antibody (Catalog # FAB4398F, filled histogram) or isotype control antibody (Catalog # IC005F, open histogram). View our protocol for Staining Membrane-associated Proteins.