| 货号 | FAB9294R-025UG |
| 别名 | CD71 antigen; CD71; CD71TRFR; p90; T9; TfR (Transferrin R); TfR (TransferrinR); TfR; TFR1; TFRC; TR; transferrin receptor (p90, CD71); transferrin receptor protein 1; TRFR | 全称 | Transferrin Receptor |
| 应用 | Flow Cytometry |
| 目标/特异性 | Detects rat TfR (Transferrin R) in flow cytometry. |
| 使用方法 | Flow Cytometry: 0.5 µg/106cells |
| 来源 | The product is shipped with polar packs. Upon receipt, store it immediately at the temperature recommended below. |
| 产品组分 |
| 供应商 | R&D Systems |
| Entrez Gene IDs | 7037 (Human); 22042 (Mouse); 64678 (Rat) |
| 纯化方式 | Protein A or G purified from hybridoma culture supernatant |
| 免疫原 | PHA activated rat lymphocytes |
| 生物活性 | Rat |
| 标记 | Alexa Fluor 647 |
| 背景 | The Transferrin Receptor (TfR or TfR-1, designated CD71) is a type 2 transmembrane glycoprotein expressed on erythroid progenitors, muscle cells and proliferating cells as a 188 kDa disulfide-linked homodimer of 95 kDa monomers. As the major mediator of cellular iron uptake, it binds and internalizes diferric transferrin, allowing iron release at the low pH of the endosome. Most soluble TfR (sTfR) arises from trypsin proteolysis at aa 100, producing the circulating form of TfR. sTfR concentration in plasma or serum is proportional to total TfR and can be increased by iron deficiency. Erythroid progenitors, which use iron for hemoglobin synthesis, normally account for the bulk of total body TfR production. Since rapidly growing cells require iron to replicate DNA, cancer cells can express up to 5-fold more TfR than quiescent cells in the surrounding tissue. Antibody targeting of TfR can inhibit tumor cell proliferation and induce apoptosis. The hereditary hemochromatosis protein HFE competes with diferric transferrin for binding to TfR, and targets TfR for degradation rather than recycling. TfR has been reported to have ferritin-independent functions in T cell development, immunological synapse formation and galectin-3-mediated cell death, and to be a cell entry receptor for New World hemorrhagic fever arenaviruses. |
| 运输条件 | Blue Ice |
| 存放说明 | 4℃ |
| 参考文献 |
|
Detection of TfR (Transferrin R) in Rat Splenocytes by Flow Cytometry. Rat splenocytes were stained with Mouse Anti-Rat TfR (Transferrin R) Alexa Fluor® 647‑conjugated Monoclonal Antibody (Catalog # FAB9294R, filled histogram) or isotype control antibody (Catalog # IC003R, open histogram). View our protocol for Staining Membrane-associated Proteins. | |
Detection of TfR (Transferrin R) in PC‑12 Rat Cell Line by Flow Cytometry. PC‑12 rat adrenal pheochromocytoma cell line was stained with Mouse Anti-Rat TfR (Transferrin R) Alexa Fluor® 647‑conjugated Monoclonal Antibody (Catalog # FAB9294R, filled histogram) or isotype control antibody (Catalog # IC003R, open histogram). View our protocol for Staining Membrane-associated Proteins. |
