| 货号 | FAB1959G-025 |
| 别名 | IL-12 receptor beta 2; IL-12 receptor subunit beta-2; IL-12R subunit beta-2; IL12RB2; IL-12RB2; IL-12R-beta-2; interleukin 12 receptor, beta 2; interleukin-12 receptor beta-2 chain; interleukin-12 receptor subunit beta-2 | 全称 | Interleukin 12 Receptor beta 2 |
| 反应种属 | Human/Mouse |
| 应用 | Flow Cytometry(10 µL/106cells) |
| 目标/特异性 | Detects human and mouse IL-12 R beta 2 in direct ELISAs. In direct ELISAs, no cross-reactivity with recombinant human IL-1 R beta 1 is observed. |
| 使用方法 | Flow Cytometry: 10 µL/106cells |
| 来源 | The product is shipped with polar packs. Upon receipt, store it immediately at the temperature recommended below. |
| 产品组分 |
| 供应商 | R&D Systems |
| Entrez Gene IDs | 3595 (Human); 16162 (Mouse) |
| 应用文献 | |
| R&D Systems personnel manually curate a database that contains references using R&D Systems products. The data collected includes not only links to publications in PubMed, but also provides information about sample types, species, and experimental conditions. Enhanced acid sphingomyelinase activity drives immune evasion and tumor growth in non-small cell lung carcinoma | |
| 纯化方式 | Protein A or G purified from hybridoma culture supernatant |
| 免疫原 | Mouse myeloma cell line NS0-derived recombinant human IL-12 R beta 2 Cys28-Asn622 Accession # Q99665 |
| 生物活性 | Human, Mouse |
| 标记 | Alexa Fluor 488 |
| 背景 | Interleukin 12 (IL-12), the founding member of the IL-12 family of heterodimeric cytokines, is composed of two disulfide-linked 35 kDa and 40 kDa subunits. The 35 kDa subunit (p35) is a alpha -helical protein homologous to IL-6 and G-CSF. The 40 kDa subunit (p40) contains one fibronectin type III and one Ig C2-like domain, and has a high degree of structural homology to type I cytokine receptors. Whereas p35 subunit is unique to IL-12, the p40 subunit is also a subunit of IL-23. IL-12 is an essential mediator of cellular-immunity that induces T cells and natural-killer cells to produce IFN-gamma. It is also required for the expansion and activation Th1 cells (1, 2). The biological activities of IL-12 are mediated through the high-affinity receptor complex composed of the IL-12 Receptor beta 1 (IL-12 R beta 1) and IL-12 Receptor beta 2 (IL‑12 R beta 2) subunits. IL-12 R beta 1 is a 100 kDa protein that is also a subunit of the IL-23 receptor complex. It binds IL-12/IL-23 p40 and is associated with Tyk2. IL‑12 R beta 2 is a 130 kDa protein that interacts with p35 and is associated with Jak2. Both receptor subunits are type I membrane proteins that share similarities with the gp130/G-CSF R subgroup in the cytokine receptor superfamily. IL-12 R beta 2 cDNA encodes a 862 amino acid (aa) protein with a putative 27 aa signal peptide that is cleaved to generate the mature protein with a 595 aa extracellular domain, a 24 aa transmembrane domain and a 216 aa cytoplasmic region. Human and mouse IL‑12 R beta 2 share 68% amino acid sequence identity. Whereas IL-12 R beta 1 expression has been detected in activated T cells, NK cells and B cells, the expression of IL‑12 R beta 2 is more restricted. Among T cells, IL-12 R beta 2 is absent on naive T cells. Activation of T cells via TCR up‑regulates IL‑12 R beta 2 expression on human Th1 but not Th2 cells (1‑4). |
| 运输条件 | Blue Ice |
| 存放说明 | 4℃ |
| 参考文献 |
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Detection of IL‑12 R beta 2 in Human PBMCs by Flow Cytometry. Human peripheral blood mononuclear cells (PBMCs) either (A) untreated or (B) treated with 5 ng/mL Recombinant Human IL‑12 (Catalog # 219-IL) and 10 μg/mLGoat Anti-Human IL‑4 Polyclonal Antibody (Catalog # AB-204-NA) for 3 days to induce Th1 development were stained with Mouse Anti-Human/Mouse IL‑12 R beta 2 Alexa Fluor® 488‑conjugated Monoclonal Antibody (Catalog # FAB1959G) and Mouse Anti-Human CD4 APC‑conjugated Monoclonal Antibody (Catalog # FAB3791A). Quadrant markers were set based on control antibody staining (Catalog # IC002G). View our protocol for Staining Membrane-associated Proteins. |
