Human/Mouse SSEA-4 Allophycocyanin MAb (Cl MC-813-70) (25 TESTS)【科瑞奥博】

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Human/Mouse SSEA-4 Allophycocyanin MAb (Cl MC-813-70) (25 TESTS)

货号： FAB1435A-025 基本售价： 2399.3 元规格： 25 Tests

产品信息

概述
货号	FAB1435A-025
别名	SSEA4; SSEA-4
全称	Stage-specific Embryonic Antigen-4
反应种属	Human/Mouse
应用	Flow Cytometry
目标/特异性	Recognizes a carbohydrate epitope of SSEA-4 (1, 2).
使用方法	Flow Cytometry: 10 µL/10⁶cells
来源	The product is shipped with polar packs. Upon receipt, store it immediately at the temperature recommended below.
产品组分

性能
供应商	R&D Systems
应用文献
R&D Systems personnel manually curate a database that contains references using R&D Systems products. The data collected includes not only links to publications in PubMed, but also provides information about sample types, species, and experimental conditions. Prospectively Isolated Human Bone Marrow Cell-Derived MSCs Support Primitive Human CD34-Negative Hematopoietic Stem Cells. Authors: Matsuoka Y, Nakatsuka R, Sumide K, Kawamura H, Takahashi M, Fujioka T, Uemura Y, Asano H, Sasaki Y, Inoue M, Ogawa H, Takahashi T, Hino M, Sonoda Y Stem Cells, 2015;33(5):1554-65. Species: Human Sample Type: Whole Cells Application: Flow JMJD5 regulates cell cycle and pluripotency in human embryonic stem cells. Authors: Zhu H, Hu S, Baker J Stem Cells, 2014;32(8):2098-110. Species: Human Sample Type: Whole Cells Application: Flow Human embryonic stem cell-derived mesenchymal stroma cells (hES-MSCs) engraft in vivo and support hematopoiesis without suppressing immune function: implications for off-the shelf ES-MSC therapies. Authors: Li O, Tormin A, Sundberg B, Hyllner J, Le Blanc K, Scheding S PLoS ONE, 2013;8(1):e55319. Species: Human Sample Type: whole cells Application: Flow High concentration of synthetic serum, stepwise equilibration and slow cooling as an efficient technique for large-scale cryopreservation of human embryonic stem cells. Authors: Lee JY, Lee JE, Kim DK, Yoon TK, Chung HM, Lee DR Fertil. Steril., 2010;93(3):976-85. Species: Human Sample Type: Whole Cells Application: Flow Evaluation of ABCG2 expression in human embryonic stem cells: crossing the same river twice? Authors: Sarkadi B, Orban TI, Szakacs G, Varady G, Schamberger A, Erdei Z, Szebenyi K, Homolya L, Apati A Stem Cells, 2010;28(1):174-6. Species: Human Sample Type: Whole Cells Application: Flow Expression and functional analysis of G1 to S regulatory components reveals an important role for CDK2 in cell cycle regulation in human embryonic stem cells. Authors: Neganova I, Zhang X, Atkinson S, Lako M Oncogene, 2009;28(1):20-30. Species: Human Sample Type: Whole Cells Application: Flow
纯化方式	Protein A or G purified from hybridoma culture supernatant
免疫原	2120Ep human embryonal carcinoma cell line
生物活性	Human, Mouse
标记	Allophycocyanin
背景	SSEA-4 is expressed on the surface of human embryonal carcinoma (EC) cells (the pluripotent stem cells of teratocarcinomas), human embryonic germ cells (EG), and human embryonic stem cells (ES). Expression of SSEA-4 is down-regulated following differentiation of human EC cells. In contrast, the differentiation of murine EC and ES cells may be accompanied by an increase in SSEA-4 expression (1-4).
运输条件	Blue Ice
存放说明	4℃
参考文献	Shevinsky, L.H. et al. (1982) Cell 30:697. Kannagi, R. et al. (1983) EMBO J. 2:2355. Thomson, J.A. and J.S. Odorico (2000) Trends Biotechnol. 18:53. Draper, J.S. et al. (2002) J. Anat. 200:249.

参考图片

Detection of SSEA‑4 in NTera‑2 Human Cell Line by Flow Cytometry. NTera‑2 human testicular embryonic carcinoma cell line was stained with Mouse Anti-Human/Mouse SSEA‑4 APC‑conjugated Monoclonal Antibody (Catalog # FAB1435A, filled histogram) or isotype control antibody (Catalog # IC007A, open histogram). View our protocol for Staining Membrane-associated Proteins.