| 货号 | IC9125G-025 |
| 简介 | Recombinant Monoclonal Antibody |
| 别名 | RORC2 | 全称 | Retinoic Acid-Related Orphan Receptor gamma t |
| 应用 | Intracellular Staining by Flow Cytometry |
| 目标/特异性 | Detects recombinant human ROR gamma t/RORC2/NR1F3 by direct ELISAs and detects human and mouse ROR gamma t/RORC2/NR1F3 in flow cytometry. |
| 使用方法 | Intracellular Staining by Flow Cytometry: 5 µL/106cells |
| 来源 | The product is shipped with polar packs. Upon receipt, store it immediately at the temperature recommended below. |
| 产品组分 |
| 供应商 | R&D Systems |
| Entrez Gene IDs | 6097 (Human) |
| 纯化方式 | Protein A or G purified from cell culture supernatant |
| 免疫原 | E.coli-derived recombinant human ROR gamma t/RORC2/NR1F3 Met1-Gln100 Accession # P51449 |
| 生物活性 | Human, Mouse |
| 标记 | Alexa Fluor 488 |
| 背景 | Retinoic acid-related Orphan Receptor gamma (ROR gamma, TOR, RORC; NR1F3) is a member of the orphan nuclear receptor family. ROR gamma is expressed in the muscle, thymus, testis, pancreas, prostate, heart, and liver. ROR gamma plays a role in thymocyte development and homeostasis. RORs bind to DNA as monomers on half-site elements with 5 A/T-rich extensions. An N-terminal isoform of ROR gamma, ROR gamma t, has been shown to be specifically expressed in the thymus. |
| 运输条件 | Blue Ice |
| 存放说明 | 4℃ |
| 参考文献 |
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Detection of ROR gamma t/RORC2/NR1F3 in Human PBMCs by Flow Cytometry. Human peripheral blood mononuclear cells (PBMCs) either (A) unstimulated or (B) stimulated to induce Th17 cells were stained with Rabbit Anti-Human/Mouse ROR gamma t/RORC2/NR1F3 Alexa Fluor® 488‑conjugated Monoclonal Antibody (Catalog # IC9125G) and Mouse Anti-Human CD4 APC‑conjugated Monoclonal Antibody (Catalog # FAB3791A). Quadrant markers were set based on control antibody staining (Catalog # IC1051G). To facilitate intracellular staining, cells were fixed and permeabilized with FlowX FoxP3 Fixation & Permeabilization Buffer Kit (Catalog # FC012). View our protocol for Staining Intracellular Molecules. | |
Detection of ROR gamma t/RORC2/NR1F3 in Mouse Splenocytes by Flow Cytometry. Mouse splenocytes either (A) unstimulated or (B) stimulated to induce Th17 cells were stained with Rabbit Anti-Human/Mouse ROR gamma t/RORC2/NR1F3 Alexa Fluor® 488‑conjugated Monoclonal Antibody (Catalog # IC9125G) and Mouse Anti-Human CD4 APC‑conjugated Monoclonal Antibody (Catalog # FAB3791A). Quadrant markers were set based on control antibody staining (Catalog # IC1051G). To facilitate intracellular staining, cells were fixed and permeabilized with FlowX FoxP3 Fixation & Permeabilization Buffer Kit (Catalog # FC012). View our protocol for Staining Intracellular Molecules. |
