| 货号 | FAB6944P-025 |
| 简介 | Recombinant Monoclonal Antibody |
| 别名 | 2F1; CLEC15A; CLEC15AMAFA-LIKE; C-type lectin domain family 15 member A; C-type lectin domain family 15, member A; ITIM-containing receptor MAFA-L; killer cell lectin-like receptor subfamily G member 1; killer cell lectin-like receptor subfamily G, member 1; MAFA; MAFAL; MAFA-L; MAFA-like receptor; MAFAMAFA-2F1; mast cell function-associated antigen (ITIM-containing); Mast cell function-associated antigen; MGC13600 | 全称 | Killer Cell Lectin-like Receptor Subfamily G Member 1 |
| 反应种属 | Mouse |
| 应用 | Flow Cytometry |
| 目标/特异性 | Detects mouse KLRG1 in flow cytometry. |
| 使用方法 | Flow Cytometry: 10 µL/106cells |
| 来源 | The product is shipped with polar packs. Upon receipt, store it immediately at the temperature recommended below. |
| 产品组分 |
| 供应商 | R&D Systems |
| Entrez Gene IDs | 10219 (Human); 50928 (Mouse); 58975 (Rat) |
| 纯化方式 | Protein A or G purified from cell culture supernatant |
| 免疫原 | Chinese hamster ovary cell line CHO-derived mouse KLRG1 Glu57-Tyr188 Accession # O88713 |
| 生物活性 | Mouse |
| 标记 | Phycoerythrin |
| 背景 | KLRG1 (Killer cell Lectin-like Receptor G1), also called MAFA (Mast cell Function Associated), is an inhibitory type II transmembrane glycoprotein of the C-type lectin family, designated CLEC15A (1). Mature mouse KLRG1 consists of a 33 amino acid (aa) cytoplasmic domain with one Immunoreceptor Tyrosine-based Inhibitory Motif (ITIM), a 23 aa transmembrane segment, and a 132 aa extracellular domain (ECD) with one C-type lectin domain (CTLD) (2). Within the ECD, mouse KLRG1 shares 57% and 80% aa sequence identity with human and rat KLRG1, respectively. Alternate splicing generates additional isoforms of mouse KLRG1 that lack either the CTLD or the CTLD, transmembrane segment, and a portion of the cytoplasmic domain (3). KLRG1 is expressed as a 30-40 kDa N-glycosylated molecule that forms disulfide-linked homodimers, trimers, and tetramers (4, 5). It is expressed on subpopulations of CD8+, CD4+, regulatory, and gamma/delta T cells as well as on NK cells (2, 4, 6-8). KLRG1 is expressed on T cells found in cord blood, but it is down-regulated postnatally and is subsequently re-expressed on antigen-exposed T cells (7, 9). It is expressed by a greater proportion of CD8+ T cells in the elderly and by virus-specific CD8+ T cells during chronic virus infection (10-12). KLRG1 binds to E-, N-, and R-Cadherins, triggering ITIM-dependent KLRG1 signaling and inhibition of T cell activation (5, 13, 14). The response is bi‑directional, as KLRG1 binding to E-Cadherin on dendritic cells (DC) can induce an anti-inflammatory DC phenotype (increased IL-10 production and decreased IL-6 and TNF-alpha production) (15). |
| 运输条件 | Blue Ice |
| 存放说明 | 4℃ |
| 参考文献 |
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Detection of KLRG1 in Mouse Splenocytes by Flow Cytometry. Mouse splenocytes were stained with Mouse Anti-Mouse CD161/NK1.1 APC‑conjugated Monoclonal Antibody (Catalog # FAB8319A) and either (A) Rabbit Anti-Mouse KLRG1 PE‑conjugated Monoclonal Antibody (Catalog # FAB6944P) or (B) Normal Rabbit IgG Phycoerythrin Control (Catalog # IC105P). View our protocol for Staining Membrane-associated Proteins. |
