Mouse CD177 Alexa Fluor 647 MAb (Clone 1171A) (25 TESTS)
货号：
FAB8186R-025 基本售价：
2250.6 元 规格：
25 Tests
产品信息
概述| 货号 | FAB8186R-025 |
| 简介 | Recombinant Monoclonal Antibody |
| 别名 | CD177 antigenNB1 GP; CD177 molecule; CD177; HNA-2a; HNA2Acell surface receptor; Human neutrophil alloantigen 2a; NB1 GP; NB1PRV-1; polycythemia rubra vera 1; Polycythemia rubra vera protein 1; PRV1; PRV1NB1 glycoprotein |
全称 | Polycythemia Rubra Vera Protein 1 | | 反应种属 | Mouse |
| 应用 | Flow Cytometry |
| 目标/特异性 | Detects mouse CD177 in direct ELISAs. |
| 使用方法 | Flow Cytometry: 5 µL/106cells
|
| 来源 | The product is shipped with polar packs. Upon receipt, store it immediately at the temperature recommended below. |
| 产品组分 | |
性能| 供应商 | R&D Systems |
| Entrez Gene IDs | 57126 (Human); 68891 (Mouse) |
| 纯化方式 | Protein A or G purified from cell culture supernatant |
| 免疫原 | Human embryonic kidney cell line HEK293-derived recombinant mouse CD177
Accession # Q8R2S8 |
| 生物活性 | Mouse |
| 标记 | Alexa Fluor 647 |
| 背景 | CD177 is a member of the uPAR/CD59/Ly6 superfamily (1). Mature mouse CD177 is a 796 amino acid (aa) protein that contains four uPAR/Ly6 domains, while human CD177 contains only two. Within common regions, mouse CD177 shares 55% and 77% aa sequence identity with human and rat CD177, respectively. CD177 is expressed on the surface of neutrophils through a glycosylphosphatidylinositol (GPI) anchor (2-4). It is nearly absent from neutrophils from paroxysmal nocturnal hemoglobinurea patients who are deficient in the ability to synthesize GPI linkages (4, 5). It is up-regulated on granulocytes from polycythemia vera and thalassemia patients (6, 7). CD177 binds to PECAM-1 on vascular endothelial cells, an interaction which mediates neutrophil adhesion to the vascular wall and neutrophil transmigration (8). It associates in cis with the Integrin MAC-1 (CD11b/CD18) (9). CD177 also associates in cis with Proteinase 3 (PR3) and is required for cell surface PR3 expression (9-11). PR3 is normally found in intracellular vesicles, but once at the cell surface it can serve as an autoimmune target for anti-neutrophil cytoplasmic antibodies (ANCA) (12). The ANCA targeting of CD177-PR3 complexes triggers neutrophil activation and vasculitis (9, 12). |
| 运输条件 | Blue Ice |
| 存放说明 | 4℃ |
| 参考文献 | - Stroncek, D.F. (2007) Curr. Opin. Hematol. 14:688.
- Skubitz, K.M. et al. (1991) J. Leukoc. Biol. 49:163.
- Kissel, K. et al. (2001) Eur. J. Immunol. 31:1301.
- Klippel, S. et al. (2002) Blood 100:2441.
- Boccuni, P. et al. (2000) Crit. Rev. Oncol. Hematol. 33:25.
- Temerinac, S. et al. (2000) Blood 95:2569.
- Zoi, K. et al. (2008) Brit. J. Haematol. 141:100.
- Sachs, U.J.H. et al. (2007) J. Biol. Chem. 282:23603.
- Jerke, U. et al. (2011) J. Biol. Chem. 286:7070.
- von Vietinghoff, S. et al. (2007) Blood 109:4487.
- Kuckleburg, C.J. et al. (2012) J. Immunol. 188:2419.
- van Timmeren, M.M. and P. Heeringa (2012) Curr. Opin. Rheumatol. 24:8.
|
参考图片Detection of CD177 in Mouse Bone Marrow Cells by Flow Cytometry. Mouse bone marrow cells were stained with Rat Anti-Mouse Gr‑1/Ly‑6G Fluorescein‑conjugated Monoclonal Antibody (Catalog # FAB1037F) and either (A) Rabbit Anti-Mouse CD177 Alexa Fluor® 647‑conjugated Monoclonal Antibody (Catalog # FAB8186R) or (B) Normal Rabbit IgG Alexa Fluor 647 Control. View our protocol for Staining Membrane-associated Proteins. |
