货号 | IC2018P-025 |
别名 | ANOP3; MCOPS3; MGC2413; SRY (sex determining region Y)-box 2; SRY-related HMG-box gene 2; transcription factor SOX2; transcription factor SOX-2 | 全称 | Transcription Factor SOX2 |
反应种属 | Human/Mouse |
应用 | Intracellular Staining by Flow Cytometry(10 µL/106cells) |
目标/特异性 | Detects human and mouse SOX2 in Western blots. |
使用方法 | Intracellular Staining by Flow Cytometry: 10 µL/106cells |
来源 | The product is shipped with polar packs. Upon receipt, store it immediately at the temperature recommended below. |
产品组分 |
供应商 | R&D Systems |
Entrez Gene IDs | 6657 (Human) |
应用文献 | |
R&D Systems personnel manually curate a database that contains references using R&D Systems products. The data collected includes not only links to publications in PubMed, but also provides information about sample types, species, and experimental conditions. A single-cell and feeder-free culture system for monkey embryonic stem cells. | |
纯化方式 | Protein A or G purified from hybridoma culture supernatant |
免疫原 | E. coli-derived recombinant human SOX2 Gly135-Met317 Accession # P48431 |
生物活性 | Human, Mouse |
标记 | Phycoerythrin |
背景 | SOX2 belongs to the SOX (SRY-like HMG box) family of transcription factors with diverse roles in development. SOX2 functions in specifying the first three lineages present at implantation and in regulating proliferation and differentiation in the developing peripheral nervous system (1-6). |
运输条件 | Blue Ice |
存放说明 | 4℃ |
参考文献 |
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Detection of SOX2 in NTera‑2 Human Cell Line by Flow Cytometry. NTera‑2 human testicular embryonic carcinoma cell line was stained with Mouse Anti-Human/Mouse SOX2 PE‑conjugated Monoclonal Antibody (Catalog # IC2018P, filled histogram) or isotype control antibody (Catalog # IC003P, open histogram). To facilitate intracellular staining, cells were fixed with Flow Cytometry Fixation Buffer (Catalog # FC004) and permeabilized with Flow Cytometry Permeabilization/Wash Buffer I (Catalog # FC005). View our protocol for Staining Intracellular Molecules. |