Human/Mouse Oct-3/4 Fluorescein MAb (Clone 240408) (25 TESTS)【科瑞奥博】

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Human/Mouse Oct-3/4 Fluorescein MAb (Clone 240408) (25 TESTS)

货号： IC1759F-025 基本售价： 2111.7 元规格： 25 Tests

产品信息

概述
货号	IC1759F-025
别名	Otf3g; Otf4; Pou5f1
全称	Octamer-binding Protein 3/4
反应种属	Human/Mouse
应用	Intracellular Staining by Flow Cytometry(10 µL/10⁶cells)
目标/特异性	Detects human Oct-3/4 in Western blots and detects mouse Oct-3/4 in flow cytometry.
使用方法	Intracellular Staining by Flow Cytometry: 10 µL/10⁶cells
来源	The product is shipped with polar packs. Upon receipt, store it immediately at the temperature recommended below.
产品组分

性能
供应商	R&D Systems
Entrez Gene IDs	5460 (Human); 18999 (Mouse)
应用文献
R&D Systems personnel manually curate a database that contains references using R&D Systems products. The data collected includes not only links to publications in PubMed, but also provides information about sample types, species, and experimental conditions. Automated, scalable culture of human embryonic stem cells in feeder-free conditions. Authors: Thomas RJ, Anderson D, Chandra A, Smith NM, Young LE, Williams D, Denning C Biotechnol. Bioeng., 2009;102(6):1636-44. Species: Human Sample Type: Whole Cells Application: Flow
纯化方式	Protein A or G purified from hybridoma culture supernatant
免疫原	E. coli-derived recombinant human Oct‑3/4 Met1-Asn265 (Met262Leu) Accession # Q01860
生物活性	Human, Mouse
标记	Fluorescein
背景	Oct-3/4, also termed Oct-3 or Oct-4, is a POU transcription factor that is expressed in totipotent embryonic stem and germ cells.(2, 3) Oct-3/4 is required to sustain stem cell self-renewal and pluripotency.(4) It is considered a master regulator of pluripotency that controls lineage commitment and is the most widely recognized marker of totipotent embryonic stem cells.
运输条件	Blue Ice
存放说明	4℃
参考文献	Takeda, J. et al. (1992) Nucleic Acids Res. 20:4613. Scholer, H.R. et al. (1989) EMBO J. 8:2543. Rosner, M.H. et al. (1990) Nature 345:686. Niwa, H. et al. (2000) Nat. Genet. 24:372.

参考图片

Detection of Oct‑3/4 in BG01V Human Cells by Flow Cytometry. BG01V human embryonic stem cells were stained with Rat Anti-Human/Mouse Oct‑3/4 Fluorescein‑conjugated Monoclonal Antibody (Catalog # IC1759F, filled histogram) or isotype control antibody (Catalog # IC013F, open histogram). To facilitate intracellular staining, cells were fixed with Flow Cytometry Fixation Buffer (Catalog # FC004) and permeabilized with Flow Cytometry Permeabilization/Wash Buffer I (Catalog # FC005). View our protocol for Staining Intracellular Molecules.

Detection of Oct‑3/4 in Mouse Embryonic Stem Cells by Flow Cytometry. Mouse embryonic stem cells treated with 5 μM Retinoic Acid for 2 days were stained with Rat Anti-Human/Mouse Oct‑3/4 Fluorescein‑conjugated Monoclonal Antibody (Catalog # IC1759F, filled histogram) or isotype control antibody (Catalog # IC013F, open histogram). To facilitate intracellular staining, cells were fixed with Flow Cytometry Fixation Buffer (Catalog # FC004) and permeabilized with Flow Cytometry Permeabilization/Wash Buffer I (Catalog # FC005). View our protocol for Staining Intracellular Molecules.