| 货号 | IC1759A-025 |
| 别名 | Otf3g; Otf4; Pou5f1 | 全称 | Octamer-binding Protein 3/4 |
| 反应种属 | Human/Mouse |
| 应用 | Intracellular Staining by Flow Cytometry(10 µL/106cells) |
| 目标/特异性 | Detects human Oct-3/4 in Western blots and detects mouse Oct-3/4 in flow cytometry. |
| 使用方法 | Intracellular Staining by Flow Cytometry: 10 µL/106cells |
| 来源 | The product is shipped with polar packs. Upon receipt, store it immediately at the temperature recommended below. |
| 产品组分 |
| 供应商 | R&D Systems |
| Entrez Gene IDs | 5460 (Human); 18999 (Mouse) |
| 应用文献 | |
| R&D Systems personnel manually curate a database that contains references using R&D Systems products. The data collected includes not only links to publications in PubMed, but also provides information about sample types, species, and experimental conditions. Murine cytomegalovirus infection of neural stem cells alters neurogenesis in the developing brain. | |
| 纯化方式 | Protein A or G purified from hybridoma culture supernatant |
| 免疫原 | E. coli-derived recombinant human Oct‑3/4 Met1-Asn265 (Met262Leu) Accession # Q01860 |
| 生物活性 | Human, Mouse |
| 标记 | Allophycocyanin |
| 背景 | Oct-3/4, also termed Oct-3 or Oct-4, is a POU transcription factor that is expressed in totipotent embryonic stem and germ cells.(2, 3) Oct-3/4 is required to sustain stem cell self-renewal and pluripotency.(4) It is considered a master regulator of pluripotency that controls lineage commitment and is the most widely recognized marker of totipotent embryonic stem cells. |
| 运输条件 | Blue Ice |
| 存放说明 | 4℃ |
| 参考文献 |
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Detection of Oct‑3/4 in BG01V Human Cells by Flow Cytometry. BG01V human embryonic stem cells were stained with Rat Anti-Human/Mouse Oct‑3/4 APC‑conjugated Monoclonal Antibody (Catalog # IC1759A, filled histogram) or isotype control antibody (Catalog # IC013A, open histogram). To facilitate intracellular staining, cells were fixed with Flow Cytometry Fixation Buffer (Catalog # FC004) and permeabilized with Flow Cytometry Permeabilization/Wash Buffer I (Catalog # FC005). View our protocol for Staining Intracellular Molecules. | |
Detection of Oct‑3/4 in D3 Mouse Cell Line by Flow Cytometry. D3 mouse embryonic stem cell line treated with 5 μM Retinoic Acid for 3 days was stained with Rat Anti-Human/Mouse Oct‑3/4 APC‑conjugated Monoclonal Antibody (Catalog # IC1759A, filled histogram) or isotype control antibody (Catalog # IC013A, open histogram). To facilitate intracellular staining, cells were fixed with Flow Cytometry Fixation Buffer (Catalog # FC004) and permeabilized with Flow Cytometry Permeabilization/Wash Buffer I (Catalog # FC005). View our protocol for Staining Intracellular Molecules. |
